Screening and separation of α-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography

被引：13

作者：

Cen, Yin ^{[1
]}

Xiao, Aiping ^{[2
]}

Chen, Xiaoqing ^{[1
]}

Liu, Liangliang ^{[2
]}

机构：

[1] Cent S Univ, Coll Chem & Chem Engn, Changsha, Hunan, Peoples R China

[2] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha, Hunan, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2017年 / 40卷 / 24期

基金：

中国国家自然科学基金;

关键词：

alpha-amylase; high-speed counter-current chromatography; magnetic graphene oxide; traditional Chinese medicine; TRADITIONAL CHINESE MEDICINE; SOLID-PHASE EXTRACTION; PREPARATIVE SEPARATION; INDUCED HYPERTHERMIA; IDENTIFICATION; STRATEGY; METABOLITES; GLYCOSIDES; DECOCTION; CANCER;

D O I：

10.1002/jssc.201700333

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A screening method using alpha-amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography was proposed and utilized to screen and separate alpha-amylase inhibitors from extract of Solanum nigrum. The alpha-amylase-functionalized magnetic graphene oxide was characterized and found to demonstrate satisfactory structure, magnetic response (24.5 emu/g), and reusability (retained 90% of initial activity after five cycles). The conditions for the screening with alpha-amylase functionalized magnetic graphene oxide were optimized and set at pH 7.0 and 25 degrees C. As a result, two potent flavonoid compounds, apigenin-7-O-glucuronide (1) and astragalin (2), were separated and collected through high-speed countercurrent chromatography and subjected to high-performance liquid chromatography analysis with purity higher than 90% (according to HPLC data), which were identified as alpha-amylase inhibitors. These results suggested that utilization of alpha-amylase functionalized magnetic graphene oxide in the rapid screening and isolation bioactive compounds from complex natural products is a feasible and environmentally friendly method.

引用

页码：4780 / 4787

页数：8

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