Genome-Guided Analysis of Physiological Capacities of Tepidanaerobacter acetatoxydans Provides Insights into Environmental Adaptations and Syntrophic Acetate Oxidation

被引:21
作者
Muller, Bettina [1 ]
Manzoor, Shahid [2 ,3 ]
Niazi, Adnan [2 ]
Bongcam-Rudloff, Erik [2 ]
Schnurer, Anna [1 ]
机构
[1] Swedish Univ Agr Sci, Bioctr, Dept Microbiol, Uppsala, Sweden
[2] Swedish Univ Agr Sci, SLU Global Bioinformat Ctr, Dept Anim Breeding & Genet Sci, Uppsala, Sweden
[3] Univ Punjab, Lahore, Pakistan
来源
PLOS ONE | 2015年 / 10卷 / 03期
关键词
CITRIC-ACID CYCLE; METHANE PRODUCTION; SP NOV; GEN; NOV; BACTERIUM; SEQUENCE; PATHWAY; SYSTEM; METABOLISM; TRANSPORT;
D O I
10.1371/journal.pone.0121237
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
This paper describes the genome-based analysis of Tepidanaerobacter acetatoxydans strain Re1, a syntrophic acetate-oxidising bacterium (SAOB). Principal issues such as environmental adaptations, metabolic capacities, and energy conserving systems have been investigated and the potential consequences for syntrophic acetate oxidation discussed. Briefly, in pure culture, T. acetatoxydans grows with different organic compounds and produces acetate as the main product. In a syntrophic consortium with a hydrogenotrophic methanogen, it can also reverse its metabolism and instead convert acetate to formate/H-2 and CO2. It can only proceed if the product formed is continuously removed. This process generates a very small amount of energy that is scarcely enough for growth, which makes this particular syntrophy of special interest. As a crucial member of the biogas-producing community in ammonium-rich engineered AD processes, genomic features conferring ammonium resistance, bacterial defense, oxygen and temperature tolerance were found, as well as attributes related to biofilm formation and flocculation. It is likely that T. acetatoxydans can form an electrochemical gradient by putative electron-bifurcating Rnf complex and [Fe-Fe] hydrogenases, as observed in other acetogens. However, genomic deficiencies related to acetogenic metabolism and anaerobic respiration were discovered, such as the lack of formate dehydrogenase and F1F0 ATP synthase. This has potential consequences for the metabolic pathways used under SAO and non-SAO conditions. The two complete sets of bacteriophage genomes, which were found to be encoded in the genome, are also worthy of mention.
引用
收藏
页数:21
相关论文
共 89 条
[21]   INTERMEDIARY METABOLISM IN CLOSTRIDIUM-ACETOBUTYLICUM - LEVELS OF ENZYMES INVOLVED IN THE FORMATION OF ACETATE AND BUTYRATE [J].
HARTMANIS, MGN ;
GATENBECK, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (06) :1277-1283
[22]   Thermacetogenium phaeum gen. nov., sp nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium [J].
Hattori, S ;
Kamagata, Y ;
Hanada, S ;
Shoun, H .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1601-1609
[23]   Operation of the CO dehydrogenase/acetyl coenzyme A pathway in both acetate oxidation and acetate formation by the syntrophically acetate-oxidizing bacterium Thermacetogenium phaeum [J].
Hattori, S ;
Galushko, AS ;
Kamagata, Y ;
Schink, B .
JOURNAL OF BACTERIOLOGY, 2005, 187 (10) :3471-3476
[24]   Involvement of formate as an interspecies electron carrier in a syntrophic acetate-oxidizing anaerobic microorganism in coculture with methanogens [J].
Hattori, S ;
Luo, HW ;
Shoun, H ;
Kamagata, Y .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2001, 91 (03) :294-298
[25]   Chemosensory signaling systems that control bacterial survival [J].
He, Kuang ;
Bauer, Carl E. .
TRENDS IN MICROBIOLOGY, 2014, 22 (07) :389-398
[26]   A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels [J].
Hickman, JW ;
Tifrea, DF ;
Harwood, CS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (40) :14422-14427
[27]   CRISPR/Cas, the Immune System of Bacteria and Archaea [J].
Horvath, Philippe ;
Barrangou, Rodolphe .
SCIENCE, 2010, 327 (5962) :167-170
[28]   A monocarboxylate permease of Rhizobium leguminosarum is the first member of a new subfamily of transporters [J].
Hosie, AHF ;
Allaway, D ;
Poole, PS .
JOURNAL OF BACTERIOLOGY, 2002, 184 (19) :5436-5448
[29]   ELECTRON-TRANSPORT AND ELECTROCHEMICAL PROTON GRADIENT IN MEMBRANE-VESICLES OF CLOSTRIDIUM-THERMOAUTOTROPHICUM [J].
HUGENHOLTZ, J ;
LJUNGDAHL, LG .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2873-2875
[30]   Prodigal: prokaryotic gene recognition and translation initiation site identification [J].
Hyatt, Doug ;
Chen, Gwo-Liang ;
LoCascio, Philip F. ;
Land, Miriam L. ;
Larimer, Frank W. ;
Hauser, Loren J. .
BMC BIOINFORMATICS, 2010, 11