Significant improvement of quality for long oligonucleotides by using controlled pore glass with large pores

被引：5

作者：

Kozlov, IA ^{[1
]}

Dang, M ^{[1
]}

Sikes, K ^{[1
]}

Kotseroglou, T ^{[1
]}

Barker, DL ^{[1
]}

Zhao, CF ^{[1
]}

机构：

[1] Illumina Inc, San Diego, CA 92121 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 5-7期

关键词：

oligonucleotide synthesis; solid support; controlled pore glass;

D O I：

10.1081/NCN-200059761

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A key factor influencing the quality of long oligonucleotides is the choice Of controlled pore glass (CPG) which is used as a solid support during oligonucleolide synthesis. We studied the influence of CPG pore size on the quality of 75-mer oligonucleotides. Using electrophoresis and HPLC, we demonstrated failure modes that can occur at certain oligo lengths with 1000A pore size, and compared yield and purity of 75-mer oligos using 1000A and larger pore size CPG. We showed that oligonucleotides with much better quality are obtained using CPG with pore sizes of 1400A and larger. We also identified the key characteristics for CPG selection that lead to the best CPG performance.

引用

页码：1037 / 1041

页数：5