Roles of ERβ and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen

被引：8

作者：

Huang, Weiren ^{[1
,2
]}

Chen, Yuanbin ^{[1
,3
,4
]}

Liu, Yuchen ^{[2
,5
]}

Zhang, Qiaoxia ^{[2
]}

Yu, Zhou ^{[1
,6
]}

Mou, Lisha ^{[1
,2
]}

Wu, Hanwei ^{[1
]}

Zhao, Li ^{[1
]}

Long, Ting ^{[3
]}

Qin, Danian ^{[3
]}

Gui, Yaoting ^{[1
]}

机构：

[1] Peking Univ, Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr,Inst Urol, Guangdong & Shenzhen Key Lab Male Reprod Med & G, Shenzhen 518036, Peoples R China

[2] Shenzhen Univ, Affiliated Hosp 1, Key Lab Med Reprogramming Technol, Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China

[3] Shantou Univ, Sch Med, Dept Physiol, Shantou 515031, Peoples R China

[4] Jiaying Univ, Coll Med, Dept Physiol, Meizhou 514031, Peoples R China

[5] Anhui Med Univ, Hefei 230032, Peoples R China

[6] Fourth Mil Med Univ, Inst Plast Surg, Xijing Hosp, Xian 710032, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2015年 / 2015卷

基金：

中国国家自然科学基金;

关键词：

RECEPTOR-ALPHA; ANDROGEN RECEPTOR; PROSTATE-CANCER; UP-REGULATION; EXPRESSION; ACTIVATION; 17-BETA-ESTRADIOL; GROWTH; INDUCTION; REGULATOR;

D O I：

10.1155/2015/251780

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bladder cancer belongs to one of the most common cancers and is a leading cause of deaths in our society. Urothelial carcinoma of the bladder (UCB) is the main type of this cancer, and the estrogen receptors in UCB remain to be studied. Our experiment aimed to investigate the possible biological effect of 17 beta-estradiol on human bladder-derived T24 carcinoma cells and to indicate its related mechanisms. T24 cells were treated with various doses of 17 beta-estradiol, and cell proliferation was detected using MTT assays. 17 beta-estradiol promoted T24 cell proliferation independent of ER beta/GPR30-regulated EGFR-MAPK pathway, while it inhibited cell growth via GPR30. Furthermore, the expression levels of downstream genes (c-FOS, BCL-2, and CYCLIN D1) were increased by 17 beta-estradiol and this effect was independently associated with activity of the EGFR-MAPK pathway. The two estrogen receptors might be potential therapeutic targets for the treatment of bladder cancer.

引用

页数：10

共 40 条

[1] G protein-coupled receptor 30 (GPR30) mediates gene expression changes and growth response to 17β-estradiol and selective GPR30 ligand G-1 in ovarian cancer cells [J].