Normal Insulin Secretion from Immune-Protected Islets of Langerhans by PEGylation and Encapsulation in the Alginate-Chitosan-PEG

被引：7

作者：

Najafikhah, Nahid ^{[1
]}

Hashemi-Najafabadi, Sameereh ^{[1
]}

Zahediasl, Saleh ^{[2
]}

Nabavimanesh, Mohammad Mahdi ^{[1
]}

Farrokhfall, Khadijeh ^{[3
]}

机构：

[1] Tarbiat Modares Univ, Fac Chem Engn, Biomed Engn Dept, Tehran, Iran

[2] Shaheed Beheshti Univ Med Sci, Res Inst Endocrine Sci, Endocrine Res Ctr, Tehran, Iran

[3] Birjand Univ Med Sci, Med Toxicol & Drug Abuse Res Ctr MTDR C, Birjand, Iran

来源：

IRANIAN JOURNAL OF BIOTECHNOLOGY | 2018年 / 16卷 / 04期

关键词：

Alginate; Chitosan; Islets of Langerhans; Insulin; Type I diabetes mellitus; PANCREATIC-ISLETS; MICROENCAPSULATED ISLETS; TRANSPLANTATION; IMMUNOISOLATION; CELLS;

D O I：

10.21859/ijb.1669

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Pancreatic islet transplantation is one of the most promising strategies for treating patients with type I diabetes mellitus. Objective: We aimed to assess the immunoisolation properties of the multilayer encapsulated islets using alginate-chitosan-PEG for immunoprotection and insulin secretion from the encapsulated islets induced under different glucose concentrations in vitro. Materials and Methods: In this study, the islets were isolated from Wistar rats. The biological function (insulin secretion) of the immunoisolated islets following to PEGylation and encapsulation in the alginate-chitosan-PEG, separately, in addition to their immuno-protection in a co-culturing with the lymphocytes isolated from the male C57BL/6 mice were investigated. respectively. Results: Alginate-chitosan-PEG decreased IL-2 secretion from the lymphocytes co-cultured with islets. Also, insulin secretion from the encapsulated and PEGylated groups was stimulated by glucose (i.e., 5.6 and 16.7 mM of glucose, respectively); showed insulin secretion similar to the naked islets, without coating, after 30 and 60 min of incubation. Conclusion: In conclusion, encapsulation and PEGylation have no negative effect on the insulin secretion and glucose sensitivity of the islets for all of the groups. Also, encapsulation decreased IL-2 secretion from the lymphocytes.

引用

页码：258 / 263

页数：6