Potent, hydroxyl radical-scavenging effect of apomorphine with iron and dopamine perfusion in rat striatum

In dopaminergic neurons, free radicals are likely produced via dopamine metabolism by monoamine oxidase or via its auto-oxidation, a process facilitated by transition metals. In this study we examined the effect and possible mechanisms of apomorphine to reduce iron- and dopamine-induced 2,3-dihydroxybenzoic acid (2,3-DHBA) formation by microdialysis. We have shown that (1) FeSO4. 7H(2)O reduced both the release of dopamine and the output of dihydroxyphenylacetic acid (DOPAC); (2) apomorphine may reduce FeSO4. 7H(2)O-induced increases of 2,3-DHBA formation; (3) apomorphine has substantially reduced DOPAC output in early phase and blocked dopamine-induced increase of 2,3-DHBA levels. It is concluded that apomorphine is a potent hydroxyl radical scavenger in vivo, especially for the dopamine formation. (C) 2001 Published by Elsevier Science B.V.