Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

被引:10
作者
Li, Chong [1 ]
Zhang, Jikai [1 ,2 ]
Xie, Haiyu [1 ]
Luo, Jingting [1 ]
Fu, Chen [1 ]
Tao, Ran [1 ]
Li, Honglang [3 ,4 ]
Fu, Yongqing [2 ]
机构
[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen Key Lab Adv Thin Films & Applicat, Shenzhen 518060, Peoples R China
[2] Northumbria Univ, Fac Engn & Environm, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England
[3] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
[4] GBA Res Innovat Inst Nanotechnol, Guangzhou 510530, Peoples R China
来源
BIOSENSORS-BASEL | 2022年 / 12卷 / 07期
基金
英国工程与自然科学研究理事会; 中国国家自然科学基金;
关键词
love wave; biosensors; CEA; AuNPs; REAL-TIME DETECTION; CANCER; CEA; IMMUNOSENSOR; GRAPHENE; BIOMARKERS; BIOSENSOR; SENSORS; FILM;
D O I
10.3390/bios12070536
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90 degrees X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2-5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.
引用
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页数:11
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