Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

被引：15

作者：

Li, Chong ^{[1
]}

Zhang, Jikai ^{[1
,2
]}

Xie, Haiyu ^{[1
]}

Luo, Jingting ^{[1
]}

Fu, Chen ^{[1
]}

Tao, Ran ^{[1
]}

Li, Honglang ^{[3
,4
]}

Fu, Yongqing ^{[2
]}

机构：

[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen Key Lab Adv Thin Films & Applicat, Shenzhen 518060, Peoples R China

[2] Northumbria Univ, Fac Engn & Environm, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England

[3] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

[4] GBA Res Innovat Inst Nanotechnol, Guangzhou 510530, Peoples R China

来源：

BIOSENSORS-BASEL | 2022年 / 12卷 / 07期

基金：

英国工程与自然科学研究理事会; 中国国家自然科学基金;

关键词：

love wave; biosensors; CEA; AuNPs; REAL-TIME DETECTION; CANCER; CEA; IMMUNOSENSOR; GRAPHENE; BIOMARKERS; BIOSENSOR; SENSORS; FILM;

D O I：

10.3390/bios12070536

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90 degrees X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2-5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

引用

页数：11

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