Loop-mediated isothermal amplification assay targeting the ompA gene for rapid detection of Riemerella anatipestifer

被引：10
|
作者：
Zheng, Fuying [1 ]
Lin, Guozhen [1 ]
Zhou, Jizhang [1 ]
Wang, Guanghua [1 ]
Cao, Xiaoan [1 ]
Gong, Xiaowei [1 ]
Qiu, Changqing [1 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Peoples R China
来源：
MOLECULAR AND CELLULAR PROBES | 2011年 / 25卷 / 01期
关键词：
LAMP; ompA gene; PCR; Riemerella anatipestifer; DUCKS; SEROTYPES; THAILAND; PROTEIN;
D O I：
10.1016/j.mcp.2010.10.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of Riemerella anatipestifer (RA) infection. The LAMP assay exhibited a higher sensitivity than conventional polymerase chain reaction (PCR) and microbial isolation. The specificity of the assay was determined by restriction enzyme digestion of the LAMP products and detection of Escherichia coli, Salmonella enterica and Pasteurella multocida. The LAMP assay was able to detect RA effectively in samples of the reference strains, isolated strains and infected duck brains. This assay is a useful tool for the diagnosis of RA infection in the clinical setting. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：65 / 67
页数：3
相关论文
共 50 条
  • [21] Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
    Myrholm, Colin L.
    Tomm, Bradley D.
    Heinzelmann, Renate
    Feau, Nicolas
    Hamelin, Richard C.
    McDougal, Rebecca
    Winkworth, Richard C.
    Ramsfield, Tod D.
    FORESTS, 2021, 12 (03):
  • [22] Molecular detection of "Candidatus Rickettsia tarasevichiae" by Loop-mediated Isothermal Amplification (LAMP) of the ompA gene
    Xue, Jing
    Ren, Qing
    Jian, Rui
    Xie, Guang-Cheng
    Chen, Yongliang
    Wang, Jiangli
    Du, Luanying
    Guo, Wen-Ping
    JOURNAL OF MICROBIOLOGICAL METHODS, 2022, 202
  • [23] Loop-mediated isothermal amplification for the rapid detection of Salmonella
    Hara-Kudo, Y
    Yoshino, M
    Kojima, T
    Ikedo, M
    FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 155 - 161
  • [24] Loop-Mediated Isothermal Amplification Assay Targeting the femA Gene for Rapid Detection of Staphylococcus aureus from Clinical and Food Samples
    Zhao Xihong
    Li, Yanmei
    Park, Myoungsu
    Wang, Jun
    Zhang, Youhong
    He, Xiaowei
    Forghani, Fereidoun
    Wang, Li
    Yu, Guangchao
    Oh, Deog-Hwan
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 23 (02) : 246 - 250
  • [25] A multiplex loop-mediated isothermal amplification assay for rapid detection of Bacillus cereus and Staphylococcus aureus
    Deng, Yanglong
    Liu, Yanquan
    Jiang, Zubin
    Wang, Jingxin
    Zhang, Qing
    Qian, Yeqian
    Yuan, Yuan
    Zhou, Xiangyu
    Fan, Guiling
    Li, Yufeng
    BIOSCIENCE TRENDS, 2019, 13 (06) : 510 - 515
  • [26] Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of lymphocystis disease virus
    Li, Qiong
    Yue, Zhiqin
    Liu, Hong
    Liang, Chengzhu
    Zheng, Xiaolong
    Zhao, Yuran
    Chen, Xiao
    Xiao, Xizhi
    Chen, Changfu
    JOURNAL OF VIROLOGICAL METHODS, 2010, 163 (02) : 378 - 384
  • [27] Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Sporisorium scitamineum in sugarcane
    Shen, W.
    Xu, G.
    Sun, L.
    Zhang, L.
    Jiang, Z.
    ANNALS OF APPLIED BIOLOGY, 2016, 168 (03) : 321 - 327
  • [28] A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene
    Wen, Junping
    Gou, Hongchao
    Zhan, Zeqiang
    Gao, Yuan
    Chen, Zhengquan
    Bai, Jie
    Wang, Shaojun
    Chen, Kaifeng
    Lin, Qijie
    Liao, Ming
    Zhang, Jianmin
    POULTRY SCIENCE, 2020, 99 (07) : 3637 - 3642
  • [29] Loop-mediated isothermal amplification (LAMP) assay for detection of Theileria sergenti infection targeting the p33 gene
    Wang, L. X.
    He, L.
    Fang, R.
    Song, Q. Q.
    Tu, P.
    Jenkins, A.
    Zhou, Y. Q.
    Zhao, J. L.
    VETERINARY PARASITOLOGY, 2010, 171 (1-2) : 159 - 162
  • [30] Detection of Peronophythora litchii on lychee by loop-mediated isothermal amplification assay
    Kong, Guanghui
    Li, Tinglu
    Huang, Weixiong
    Li, Minhui
    Shen, Wankuan
    Jiang, Liqun
    Hsiang, Tom
    Jiang, Zide
    Xi, Pinggen
    CROP PROTECTION, 2021, 139
12345