Loop-mediated isothermal amplification assay targeting the ompA gene for rapid detection of Riemerella anatipestifer

被引：10
|
作者：
Zheng, Fuying [1 ]
Lin, Guozhen [1 ]
Zhou, Jizhang [1 ]
Wang, Guanghua [1 ]
Cao, Xiaoan [1 ]
Gong, Xiaowei [1 ]
Qiu, Changqing [1 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Peoples R China
来源：
MOLECULAR AND CELLULAR PROBES | 2011年 / 25卷 / 01期
关键词：
LAMP; ompA gene; PCR; Riemerella anatipestifer; DUCKS; SEROTYPES; THAILAND; PROTEIN;
D O I：
10.1016/j.mcp.2010.10.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of Riemerella anatipestifer (RA) infection. The LAMP assay exhibited a higher sensitivity than conventional polymerase chain reaction (PCR) and microbial isolation. The specificity of the assay was determined by restriction enzyme digestion of the LAMP products and detection of Escherichia coli, Salmonella enterica and Pasteurella multocida. The LAMP assay was able to detect RA effectively in samples of the reference strains, isolated strains and infected duck brains. This assay is a useful tool for the diagnosis of RA infection in the clinical setting. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：65 / 67
页数：3
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