Development of a monoclonal antibody against Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) that can detect EBNA-LP expressed in P3HR1 cells

被引：7
作者：
Shaku, F
Matsuda, G
Furuya, R
Kamagata, C
Igarashi, M
Tanaka, M
Kanamori, M
Nishiyama, Y
Yamamoto, N
Kawaguchi, Y
机构：
[1] Nagoya Univ, Grad Sch Med, Dept Virol, Showa Ku, Nagoya, Aichi 4668550, Japan
[2] Tokyo Med & Dent Univ, Dept Cell Regulat, Med Res Inst, Bunkyo Ku, Tokyo 1138510, Japan
[3] Tokyo Med & Dent Univ, Dept Mol Virol Bioresponse, Grad Sch Med, Bunkyo Ku, Tokyo 1138510, Japan
[4] Ctr AIDS Res, Natl Inst Infect Dis, Shinjuku Ku, Tokyo 1628640, Japan
[5] PRESTO, Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2005年 / 49卷 / 05期
关键词：
EBV; EBNA-LP; P3HR1;
D O I：
10.1111/j.1348-0421.2005.tb03743.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody was mapped in the W2 domain of EBNA-LP. While another monoclonal antibody to EBNA-LP, JF186, which is widely used for analyses of the viral protein, did not react with truncated forms of EBNA-LP expressed in P3HR1 cells, as reported earlier, the LP4D3 antibody did. The LP4D3 antibody will be a useful tool for further studies of EBNA-LP, especially investigations into the phenotypes of mutant EBNA-LP expressed in P3HR1 cells.
引用
收藏
页码：477 / 483
页数：7
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