Effects of the nonsteroidal anti-inflammatory drug piroxicam on rat liver mitochondria

被引：9
作者：
SalgueiroPagadigorria, CL [1 ]
KelmerBracht, AM [1 ]
Bracht, A [1 ]
IshiiIwamoto, EL [1 ]
机构：
[1] UNIV ESTADUAL MARINGA,DEPT BIOCHEM,LAB LIVER METAB,BR-87020900 MARINGA,PARANA,BRAZIL
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1996年 / 113卷 / 01期
关键词：
piroxicam; nonsteroidal anti-inflammatories; liver; mitochondria; oxygen uptake; oxidative phosphorylation; ATPase activity; swelling; uncoupling action;
D O I：
10.1016/0742-8413(95)02041-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. The effects of piroxicam, a nonsteroidal anti-inflammatory drug, on rat liver mitochondria were investigated in order to obtain direct evidence about a possible uncoupling effect, as suggested by a previous work with the perfused rat liver. 2. Piroxicam increased respiration in the absence of exogenous ADP and decreased respiration in the presence of exogenous ADP, the ADP/O ratios and the respiratory control ratios. 3. The ATPase activity of intact mitochondria was increased by piroxicam. With 2,4-dinitrophenol uncoupled mitochondria, inhibition was observed. The ATPase activity of freeze-thawing disrupted mitochondria was insensitive to piroxicam. 4. Swelling driven by the oxidation of several substrates and safranine uptake induced by succinate oxidation were inhibited. 5. The results of this work represent a direct evidence that piroxicam acts as an uncoupler, thus, decreasing mitochondrial ATP generation.
引用
收藏
页码：85 / 91
页数：7
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