Standardization of Biochemical Profile of Mesenchymal Cell Materials by Probing the Level of Dehydrogenase Activity

被引：9

作者：

Burunova, V. V. ^{[1
]}

Gisina, A. M. ^{[2
]}

Kholodenko, I. V. ^{[1
]}

Lupatov, A. Yu. ^{[1
,2
]}

Shragina, O. A. ^{[1
]}

Yarygin, K. N. ^{[1
,2
]}

机构：

[1] Russian Acad Med Sci, Res Inst Gen Pathol & Pathophysiol, Moscow, Russia

[2] Russian Acad Med Sci, VN Orekhovich Res Inst Biomed Chem, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2010年 / 149卷 / 04期

关键词：

MTT method; primary cell cultures; mesenchymal stem cells; standardization; ASSAY;

D O I：

10.1007/s10517-010-0978-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

It is demonstrated that the output optical signal of MTT test is directly proportional to the number of viable cells in the primary culture of mesenchymal cells (skin fibroblasts and mesenchymal stem cells from the bone marrow, placenta, and umbilical cord). The slope of the best curve in coordinates "cell number - optical signal" reflecting specific productivity of MTT-formazan characterizes mean dehydrogenase activity of cells and their physiological activity. It was found that in vitro dehydrogenase activity of primary cultures of mesenchymal cells increased during the first 3-5 passages and then tended to decrease. The variant of MTT method presented here can be used for standardization of cell materials.

引用

页码：497 / 501

页数：5

共 14 条

[1]

Ahmadian Somaieh, 2009, J Vis Exp, DOI 10.3791/1191

[2]

Berridge MV, 2005, BIOTECHNOL ANN REV, V11, P127, DOI 10.1016/S1387-2656(05)11004-7

[3]

Burunov V. V., 2009, RUSS MED ZH, V17, P1058

[4]

BURUNOVA VV, 2008, KLET TEKHNOL BIOL ME, P97

[5] Appraisal of the MTT-based assay as a useful tool for predicting drug chemosensitivity in leukemia [J].

Hayon, T ;

Dvilansky, A ;

Shpilberg, O ;

Nathan, I .

LEUKEMIA & LYMPHOMA, 2003, 44 (11) :1957-1962

[6]

Loo DT, 1998, METHOD CELL BIOL, V57, P251

[7] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[8]

MURRAY R, 2004, HUMAN BIOCH

[9]

PEARSE E, 1962, HISTOCHEMISTRY THEOR

[10]

Sargent JM, 2003, RECENT RESULTS CANC, V161, P13

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