UBQLN1 interacts with SPEM1 and participates in spermiogenesis

被引：35

作者：

Bao, Jianqiang ^{[1
,2
,3
]}

Zhang, Jie ^{[4
]}

Zheng, Huili ^{[2
]}

Xu, Chen ^{[2
,3
]}

Yan, Wei ^{[1
]}

机构：

[1] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA

[2] Shanghai Jiao Tong Univ, Dept Histol & Embryol, Sch Med, Shanghai 200030, Peoples R China

[3] Shanghai Key Lab Reprod Med, Shanghai, Peoples R China

[4] China Med Univ, Dept Biochem, Shenyang, Peoples R China

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2010年 / 327卷 / 1-2期

关键词：

Spermatogenesis; Ubiquitination; Ubiquitin; Sperm; Infertility; Manchette; Protein turnover; MALE-INFERTILITY; MAMMALIAN SPERMATOGENESIS; MANCHETTE DEVELOPMENT; UBIQUITIN-BINDING; 26S PROTEASOME; SPERMATID HEAD; MUTANT MICE; PROTEIN; GENE; UBIQUILIN-1;

D O I：

10.1016/j.mce.2010.06.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Spermiogenesis represents the process through which haploid male germ cells differentiate from round spermatids into elongated spermatids and eventually the male gametes called spermatozoa. Haploid cell differentiation is unique to male germ cell development and many unique genes/proteins essential for this process have been discovered. SPEM1 is one of these spermiogenesis-essential proteins encoded by a testis-specific gene exclusively expressed in the developing spermatids Inactivation of Spem1 in mice results in deformed spermatozoa characterized by "head-bent-back" abnormalities with 100% penetrance Using yeast two-hybrid screening assays, we identified UBQLN1 as one of the SPEM1-interacting partners. UBQLN1 and SPEM1 were colocalized to the manchette of elongating spermatids Since UBQLN1 functions through binding and directing poly-ubiquitinated proteins to the proteasome for degradation, interactions between UBQLN1 and SPEM1 suggest a role in the regulation of protein ubiquitination during spermiogenesis. (C) 2010 Elsevier Ireland Ltd All rights reserved

引用

页码：89 / 97

页数：9

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