An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus

被引:3
作者
Guo, Yiwen [1 ,2 ,3 ,4 ,5 ]
Xie, Songhua [1 ,2 ,3 ,4 ,5 ]
Xu, Zhenqi [1 ,2 ,3 ,4 ,5 ]
Xie, Quan [1 ,2 ,3 ,4 ,5 ]
Wang, Weikang [1 ,2 ,3 ,4 ,5 ]
Wan, Zhimin [1 ,2 ,3 ,4 ,5 ]
Li, Tuofan [1 ,2 ,3 ,4 ,5 ]
Qin, Aijian [1 ,2 ,3 ,4 ,5 ]
Shao, Hongxia [1 ,2 ,3 ,4 ,5 ]
Ye, Jianqiang [1 ,2 ,3 ,4 ,5 ]
机构
[1] Yangzhou Univ, Minist Educ, Key Lab Avian Prevent Med, Yangzhou, Jiangsu, Peoples R China
[2] Yangzhou Univ, Key Lab Jiangsu Prevent Vet Med, Yangzhou, Jiangsu, Peoples R China
[3] Jiangsu Coinnovat Ctr Prevent & Control Importann, Yangzhou, Jiangsu, Peoples R China
[4] Yangzhou Univ, Inst Agr Sci & Technol Dev, Yangzhou, Jiangsu, Peoples R China
[5] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China
关键词
serotype 4 fowl adenovirus; virus neutralization test; recombinant virus; serological detection; high efficacy; VACCINE; PATHOGENICITY; FIBER; ELISA;
D O I
10.3389/fvets.2022.867697
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Currently, the outbreak of serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and caused tremendous economic loss to the poultry industry. Although inactivated vaccines have been licensed against FAdV-4 in China, a rapid and efficient serological method for measuring the titer of neutralizing antibodies (NAbs) specific for FAdV-4 post-infection or vaccination is rarely reported. Classical virus neutralization test (VNT) is superior in sensitivity and specificity for detecting NAbs but is either time-consuming or laborious. In this study, a recombinant virus FA4-EGFP expressing EGFP-fiber-2 fusion protein, rather than wild type (WT) FAdV-4 was used to develop a novel VNT for detecting FAdV-4 NAbs. Specificity analysis showed that the approach only reacted with the sera against FAdV-4, not with the sera against other avian pathogens tested. The novel VNT was effective in the detection of NAbs against FAdV-4 in sera from both experimentally infected and clinically vaccinated chickens, and had good linear correlation with the classical VNT. Moreover, the novel VNT not only significantly simplifies the procedure for detection of NAbs, but also shortens the timeline to 24 h in comparison with the classical VNT with 3-4 d. All these data demonstrate that the FA4-EGFP based VNT developed here provides an efficient diagnostic method for monitoring the immunological state of the vaccination or diagnosing the clinical infection of FAdV-4 in a quick and funding-saving manner.
引用
收藏
页数:7
相关论文
共 50 条
  • [31] Identification of ORF1B as a unique nonstructural protein for fowl adenovirus serotype 4
    Gao, Shenyan
    Li, Ruixue
    Zhang, Xiaozhan
    Wang, Zeng
    MICROBIAL PATHOGENESIS, 2024, 186
  • [32] Genetic evolution of fowl adenovirus serotype 4 and its pathogenicity to Cherry Valley ducks in China
    Wu, Bingrong
    Yang, Binghua
    He, Dalin
    Tang, Yi
    Diao, Youxiang
    VETERINARY MICROBIOLOGY, 2022, 274
  • [33] Development of sensitive indirect enzyme-linked immunosorbent assays for specific detection of antibodies against fowl adenovirus serotypes 1 and 4 in chickens
    Feichtner, Franziska
    Schachner, Anna
    Berger, Evelyn
    Hess, Michael
    AVIAN PATHOLOGY, 2018, 47 (01) : 73 - 82
  • [34] Pathogenicity and molecular characteristics of fowl adenovirus serotype 4 with moderate virulence in Guangxi Province, China
    Wei, You
    Xie, Zhixun
    Fan, Qing
    Xie, Zhiqin
    Deng, Xianwen
    Luo, Sisi
    Li, Xiaofeng
    Zhang, Yanfang
    Zeng, Tingting
    Huang, Jiaoling
    Ruan, Zhihua
    Wang, Sheng
    FRONTIERS IN VETERINARY SCIENCE, 2023, 10
  • [35] Pathogenic, Phylogenetic, and Serological Analysis of Group I Fowl Adenovirus Serotype 4 SDSX Isolated From Shandong, China
    Yu, Guanliu
    Wang, Yawen
    Zhang, Minmin
    Lin, Yun
    Tang, Yi
    Diao, Youxiang
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [36] Design and Identification of a Novel Antiviral Affinity Peptide against Fowl Adenovirus Serotype 4 (FAdV-4) by Targeting Fiber2 Protein
    Chen, Xiao
    Wei, Qiang
    Si, Fusheng
    Wang, Fangyu
    Lu, Qingxia
    Guo, Zhenhua
    Chai, Yongxiao
    Zhu, Rongfang
    Xing, Guangxu
    Jin, Qianyue
    Zhang, Gaiping
    VIRUSES-BASEL, 2023, 15 (04):
  • [37] Efficient cross-protection against serotype 4/8a fowl adenoviruses (FAdVs): recombinant FAdV-4 with FAdV-8a Fiber
    Lu, Yixuan
    Yuan, Yaqin
    Jiang, Huiru
    Xu, Zhenqi
    Guo, Yiwen
    Cao, Xudong
    Li, Tuofan
    Wan, Zhimin
    Shao, Hongxia
    Qin, Aijian
    Xie, Quan
    Ye, Jianqiang
    MICROBIOLOGY SPECTRUM, 2023,
  • [38] Multiantigen epitope fusion recombinant proteins from capsids of serotype 4 fowl adenovirus induce chicken immunity against avian Angara disease
    Guo, Xun
    Chang, Jiang
    Lu, Shiying
    Hu, Pan
    Zou, Deying
    Li, Yansong
    Li, Feng
    Liu, Jishan
    Cao, Qi
    Zhang, Kai
    Zhan, Junpeng
    Liu, Yixin
    Yang, Xin
    Ren, Honglin
    VETERINARY MICROBIOLOGY, 2023, 278
  • [39] An efficient double-fluorescence approach for generating fiber-2-edited recombinant serotype 4 fowl adenovirus expressing foreign gene
    Guo, Yiwen
    Xu, Zhenqi
    Chao, Yifei
    Cao, Xudong
    Jiang, Huiru
    Li, Han
    Li, Tuofan
    Wan, Zhimin
    Shao, Hongxia
    Qin, Aijian
    Xie, Quan
    Ye, Jianqiang
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [40] Genomic characterization of a fowl adenovirus serotype 4 strain isolated from a chicken with hydropericardium syndrome in Japan
    Mase, Masaji
    Tanaka, Yuko
    Iseki, Hiroshi
    Watanabe, Satoko
    ARCHIVES OF VIROLOGY, 2022, 167 (04) : 1191 - 1195