Signal transducer and activator of transcription 3 upregulates interleukin-8 expression at the level of transcription in human melanoma cells

被引：14

作者：

Oka, Masahiro ^{[1
]}

Sakaguchi, Masanobu

Okada, Taro ^{[2
]}

Nagai, Hiroshi

Ozaki, Michitaka ^{[3
]}

Yoshioka, Toyo ^{[4
]}

Inoue, Hiroshi ^{[5
]}

Mukaida, Naofumi ^{[6
,7
]}

Kikkawa, Ushio ^{[8
]}

Nishigori, Chikako

机构：

[1] Kobe Univ, Grad Sch Med, Div Dermatol, Dept Internal Related,Chuo Ku, Kobe, Hyogo 6500017, Japan

[2] Kobe Univ, Grad Sch Med, Div Biochem, Dept Mol & Cellular Biol, Kobe, Hyogo 6500017, Japan

[3] Hokkaido Univ, Sch Med, Dept Mol Surg, Sapporo, Hokkaido 060, Japan

[4] Kobe Univ, Grad Sch Med, Div Diabet Metab & Endocrinol, Dept Internal Related, Kobe, Hyogo 6500017, Japan

[5] Kanazawa Univ, Frontier Sci Org, Kanazawa, Ishikawa, Japan

[6] Kanazawa Univ, Canc Res Inst, Ctr Target Drug Dev, Kanazawa, Ishikawa 920, Japan

[7] Kanazawa Univ, Div Mol Bioregulat, Kanazawa, Ishikawa 920, Japan

[8] Kobe Univ, Biosignal Res Ctr, Kobe, Hyogo 6500017, Japan

来源：

EXPERIMENTAL DERMATOLOGY | 2010年 / 19卷 / 08期

关键词：

IL-8; luciferase assay; melanoma; STAT3; transcription; EPIDERMAL-GROWTH-FACTOR; KAPPA-B ACTIVATION; MALIGNANT-MELANOMA; CHEMOTACTIC CYTOKINES; METASTATIC MELANOMA; STAT3; ACTIVATION; REQUIREMENT; INDUCTION; PATHWAY; BINDING;

D O I：

10.1111/j.1600-0625.2009.00972.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Many melanoma cells continuously produce interleukin-8 (IL-8). The involvement of signal transducer and activator of transcription 3 (STAT3) in the constant production of IL-8 in melanoma cells was examined. The level of IL-8 production correlated well with that of the phosphorylated (activated) STAT3 in six human melanoma cell lines. Introduction of the constitutively activated form of STAT3 (STAT3-C) into WM35 melanoma cells, that show low levels of IL-8 and phosphorylated STAT3, enhanced IL-8 production. Knockdown of STAT3 suppressed IL-8 production in WM1205Lu cells that contain a high level of IL-8 accompanied by STAT3 phosphorylation. Introduction of STAT3-C markedly increased the luciferase activity in WM1205Lu cells transfected with reporter vectors linked to the 5'-flanking region of the IL-8 gene from -546 to +44 base pair (bp) and from -272 to +44 bp, but not in cells expressing reporter plasmids from -133 to +44 bp and from -98 to +44 bp. These results indicate that the upregulation of IL-8 production is caused by constitutive STAT3 activation at the level of gene transcription in melanoma cells.

引用

页码：E50 / E55

页数：6

共 48 条

[1] MOLECULAR-CLONING OF APRF, A NOVEL IFN-STIMULATED GENE FACTOR-3 P91-RELATED TRANSCRIPTION FACTOR INVOLVED IN THE GP130-MEDIATED SIGNALING PATHWAY [J].