Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties

被引：10

作者：

Cui, Jin ^{[1
,2
,3
]}

Lethu, Sebastien ^{[1
,2
,3
]}

Yasuda, Tomokazu ^{[1
]}

Matsuoka, Shigeru ^{[1
,2
,3
]}

Matsumori, Nobuaki ^{[1
]}

Sato, Fuminori ^{[2
,3
]}

Murata, Michio ^{[1
,2
,3
]}

机构：

[1] Osaka Univ, Dept Chem, Toyonaka, Osaka 5600043, Japan

[2] Osaka Univ, Lipid Active Struct Project, ERATO, JST, Toyonaka, Osaka 5600043, Japan

[3] Osaka Univ, Project Res Ctr Fundamental Sci, Toyonaka, Osaka 5600043, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2015年 / 25卷 / 02期

关键词：

Lipid raft; Phase separation; Phosphatidylcholine; Solid-state H-2 NMR; LIPID RAFTS; PHASE-DIAGRAM; DIHYDROSPHINGOMYELIN; SPHINGOMYELIN; HIV-1; HYDRATION; BILAYERS;

D O I：

10.1016/j.bmcl.2014.11.072

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-H-2(2)] oleic acid, and newly synthesized [C6-H-2(2)] DOPC. In raft-like oriented bilayers, [C6-H-2(2)] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：203 / 206

页数：4

共 4 条

[1] A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine
Minto, RE
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Lorigan, GA
CHEMISTRY AND PHYSICS OF LIPIDS, 2004, 132 (01) : 55 - 64
[2] Solid-State 2H NMR Reveals Changes in Membrane Flexibility Due to Osmotic Pressure
Mallikarjunaiah, K. J.
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BIOPHYSICAL JOURNAL, 2012, 102 (03) : 292A - 292A
[3] Solid-State 2H NMR Shows Equivalence of Dehydration and Osmotic Pressures in Lipid Membrane Deformation
Mallikarjunaiah, K. J.
Leftin, Avigdor
Kinnun, Jacob J.
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Rogozea, Adriana L.
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Brown, Michael F.
BIOPHYSICAL JOURNAL, 2011, 100 (01) : 98 - 107
[4] Hydration properties of regioselectively etherified celluloses monitored by 2H and 13C solid-state MAS NMR spectroscopy
Larsen, Flemming H.
Schoebitz, Michael
Schaller, Jens
CARBOHYDRATE POLYMERS, 2012, 89 (02) : 640 - 647

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