In vivo and in vitro anti-sepsis effects of physcion 8-O-β- glucopyranoside extracted from Rumex japonicus

The present study was designed to investigate the anti-sepsis effects of physcion 8-O-beta-glucopyranoside (POG) isolated from Rumex japonicas and explore its possible pharmacological mechanisms. POG was extracted from R. japonicas by bioactivity-guided isolation with the anti-sepsis agents. Survival analysis in septic mouse induced by LPS and heat-killed Escherichia coli were used to evaluate the protective effect of POG (40 mg.kg(-1), i.p.) on sepsis. Cytokines including TNF-alpha, IL-1 beta and IL-6 in RAW 264.7 cells induced by LPS (100 ng.mL(-1)) were determined by ELISA. In addition, the proteins expressions of TLR2 and TLR4 were determined by Western blotting assay. Our results demonstrated that POG (40 mg.kg(-1), i.p.) possessed significant protective activity on the endotoxemic mice. The POG treatment (20, 40, and 80 mu g.mL(-1)) significantly decreased the TNF-alpha, IL-1 beta and IL-6 induced by LPS (P < 0.01) in a concentration-dependent manner. Furthermore, the TLR4 and TLR2 proteins were also down-regulated by POG at 20 (P < 0.01), 40 (P < 0.01), and 80 mu g.mL(-1) (P < 0.01). The present study demonstrated that the POG extracted from R. japonicas possessed significant anti-sepsis effect on endotoxemic mice, and can be developed as a novel drug for treating sepsis in the future.