Critical Appraisal of the Side Population Assay in Stem Cell and Cancer Stem Cell Research

被引：263
作者：
Golebiewska, Anna [1 ]
Brons, Nicolaas H. C. [2 ]
Bjerkvig, Rolf [1 ,3 ]
Niclou, Simone P. [1 ]
机构：
[1] Ctr Rech Publ Sante, Dept Oncol, NorLux Neurooncol Lab, L-1526 Luxembourg, Luxembourg
[2] Ctr Rech Publ Sante, Core Facil Flow Cytometry, L-1526 Luxembourg, Luxembourg
[3] Univ Bergen, Dept Biomed, N-5009 N Bergen, Norway
来源：
CELL STEM CELL | 2011年 / 8卷 / 02期
关键词：
DRUG EFFLUX TRANSPORTERS; BLOOD-BRAIN-BARRIER; P-GLYCOPROTEIN; BONE-MARROW; NEURAL STEM; IN-VIVO; RESISTANCE PROTEIN; ABCG2; EXPRESSION; MOUSE MODEL; DYE EFFLUX;
D O I：
10.1016/j.stem.2011.01.007
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
引用
收藏
页码：136 / 147
页数：12
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