Fluorescence Decrease of Conjugated Polymers by the Catalytic Activity of Horseradish Peroxidase and Its Application in Phenolic Compounds Detection

被引：7

作者：

Gonzalez-Sanchez, M. I. ^{[1
]}

Laurenti, M. ^{[2
]}

Rubio-Retama, J. ^{[2
]}

Valero, E. ^{[1
]}

Lopez-Cabarcos, E. ^{[2
]}

机构：

[1] Univ Castilla La Mancha, Sch Ind Engn, Dept Phys Chem, Albacete 02071, Spain

[2] Univ Complutense Madrid, Fac Pharm, Dept Phys Chem, E-28040 Madrid, Spain

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 04期

关键词：

OXIDATION; MECHANISM; SENSORS; PHOTOLUMINESCENCE; ENHANCEMENT;

D O I：

10.1021/bm200091m

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the fluorescence decrease of the water-soluble pi-pi-conjugated polymer poly(2-methoxy-5-propyloxy sulfonate phenylene vinylene, MPS-PPV) by the catalytic activity of horseradish peroxidase in the presence of H2O2. MPS-PPV acts as a donor substrate in the catalytic cycle of horseradish peroxidase where the electron-deficient enzymatic intermediates compounds I and II can subtract electrons from the polymer leading to its fluorescence decrease. The addition MPS-PV of phenolic drug acetaminophen to the former solution favors the decrease of the polymer fluorescence, which indicates the peroxidase-catalyzed co-oxidation of MPS-PPV and acetaminophen. The encapsulation of horseradish peroxidase within polyacrylamide microgels allows the isolation of intermediates compound land compound II from the polymer, leading to a fluorescence decrease that is only due to the product of biocatalytic acetaminophen oxidation. This system could be used to develop a new device for phenolic compounds. detection.

引用

页码：1332 / 1338

页数：7

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