Anticoccidial and antiinflammatory activity of garlic in murine Eimeria papillata infections

被引:76
作者
Dkhil, M. A. [1 ,2 ]
Abdel-Baki, A. S. [1 ,3 ]
Wunderlich, F. [1 ,4 ]
Sies, H. [1 ,5 ]
Al-Quraishy, S. [1 ]
机构
[1] King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia
[2] Helwan Univ, Fac Sci, Dept Zool & Entomol, Cairo, Egypt
[3] Beni Suef Univ, Fac Sci, Dept Zool, Bani Suwayf, Egypt
[4] Univ Dusseldorf, Dept Mol Parasitol, D-40225 Dusseldorf, Germany
[5] Univ Dusseldorf, Dept Biochem & Mol Biol, D-40225 Dusseldorf, Germany
关键词
Garlic; Coccidiosis; Anticoccidium; Anti-inflammatory activity; RABBITS ORYCTOLAGUS-CUNICULUS; INTESTINAL COCCIDIA INFECTION; HUMAN TUMOR-CELLS; NITRIC-OXIDE; HYDROMINERAL METABOLISM; DIARRHEIC RABBITS; SERUM; EXTRACT; PLASMA; SPOROZOITES;
D O I
10.1016/j.vetpar.2010.09.009
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Coccidiosis with the protozoan parasite Eimeria as the infectious agent causes enormous economic losses, particularly in poultry farms. Here, we investigated the effects of garlic on the outcome of coccidiosis caused by Eimeria papillata in male Balb/c mice. The data showed that mice infected with E. papillata revealed an output of 3260 +/- 680 oocysts per gram faeces on day 4 p.i.. This output is significantly decreased to 1820 +/- 415 oocysts in garlic-treated mice. Infection also induced inflammation and injury of the liver. This was evidenced (i) as increases in inflammatory cellular infiltrations, dilated sinusoids, and vacuolated hepatocytes, (ii) as increased mRNA levels of inducible nitric oxide synthase (iNOS) and of the cytokines interferon gamma (IFN-gamma), and interleukin-6 (IL-6), (iii) as increased plasma levels of alanine and aspartate aminotransferases, alkaline phosphatase, gamma-glutamyl transferase and total bilirubin, (iv) as increased production of nitric oxide derived products (nitrite/nitrate) and malondialdehyde, and (v) as lowered glutathione levels and decreased activities of catalase and superoxide dismutase, respectively. All these infection-induced parameters were significantly less altered during garlic treatment. In particular, garlic counteracted the E. papillata-induced loss of glutathione and the activities of catalase and superoxide dismutase. Our data indicated that garlic treatment significantly attenuated inflammation and injury of the liver induced by E. papillata infections. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:66 / 72
页数:7
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