Virus-induced gene silencing in Solanum torvum

被引：2
|
作者：
Nakano, Masahito [1 ]
Mukaihara, Takafumi [2 ]
机构：
[1] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
[2] Res Inst Biol Sci Okayama RIBS, 7549-1 Yoshikawa,Kibichuo Cho, Okayama 7161241, Japan
来源：
JOURNAL OF GENERAL PLANT PATHOLOGY | 2022年 / 88卷 / 01期
基金：
日本学术振兴会;
关键词：
Solanum torvum; Virus-induced gene silencing; Ralstonia solanacearum; Reactive oxygen species; Salicylic acid; CHLOROPLASTS; RESISTANCE; BACTERIAL;
D O I：
10.1007/s10327-021-01040-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Solanum torvum has broad resistance to wilt-causing bacteria and fungi and nematodes. Here, we applied virus-induced gene silencing (VIGS) in S. torvum and optimized the experimental conditions to suppress a carotenoid biosynthesis gene with high efficiency. Using this optimized method, we suppressed the StPsbO and StPsbP genes, which encode components of the oxygen-evolving complex, in S. torvum. Silencing of StPsbO and StPsbP impaired defense response in S. torvum, making it more susceptible to Ralstonia solanacearum. These results indicate that the optimized VIGS method can be used to identify genes required for plant immunity in S. torvum.
引用
收藏
页码：10 / 16
页数：7
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