Quantitative Determination of Aucubin in Seven Plantago Species Using HPLC, HPTLC, and LC-ESI-MS Methods

被引：17

作者：

Jankovic, Teodora ^{[1
]}

Menkovic, Nebojsa ^{[1
]}

Zdunic, Gordana ^{[1
]}

Beara, Ivana ^{[2
]}

Balog, Kristina ^{[2
]}

Savikin, Katarina ^{[1
]}

Mimica-Dukic, Neda ^{[2
]}

机构：

[1] Inst Med Plant Res, Belgrade 11000, Serbia

[2] Univ Novi Sad, Fac Sci, Dept Chem, Novi Sad 21000, Serbia

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 16期

关键词：

Aucubin; HPLC; HPTLC; LC-ESI-MS; Plantago spp; IRIDOID GLYCOSIDES; CHROMATOGRAPHY; CATALPOL; DENSITOMETRY; CONSTITUENTS; SEPARATION; LEAVES;

D O I：

10.1080/00032711003725581

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of aucubin in Plantago lanceolata. The analyses were carried out on Zorbax SB-C18 column with an aqueous phosphoric acid and acetonitrile gradient. The correlation coefficient of calibration curve showed good linearity (r0.9995), with average recoveries between 96.7 and 104.5%. The developed method was applied for quantification in P. atrata, P. bellardii, P. coronopus, P. holosteum, P. reniformis, and P. schwarzenbergiana. The aucubin content in plant extracts was compared by HPLC, HPTLC, and LC-ESI-MS techniques and no significant differences between the conducted methods were observed.

引用

页码：2487 / 2495

页数：9

共 21 条

[1] A validation protocol for the HPTLC standardization of herbal products:: Application to the determination of acteoside in leaves of Plantago palmata Hook. f.s. [J].