mRNAs sequestered in stress granules recover nearly completely for translation

被引：14
作者：
Das, Sarada [1 ]
Santos, Leonardo [1 ]
Failla, Antonio Virgilio [2 ]
Ignatova, Zoya [1 ]
机构：
[1] Univ Hamburg, Inst Biochem & Mol Biol, Hamburg, Germany
[2] Univ Med Ctr Hamburg Eppendorf, Microscopy Imaging Facil, Hamburg, Germany
来源：
RNA BIOLOGY | 2022年 / 19卷 / 01期
关键词：
mRNA; translation; m(6)A modification; deep sequencing; mRNA structure; stress granules; SECONDARY STRUCTURES; GENE-EXPRESSION; REVEALS; TRANSCRIPTION; ACCUMULATION; PRINCIPLES; STABILITY; MICRORNA;
D O I：
10.1080/15476286.2022.2094137
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Stress granules (SGs) are membrane-less condensates composed of RNA and protein that assemble in response to stress stimuli and disassemble when stress is lifted. Both assembly and disassembly are tightly controlled processes, yet, it remains elusive whether mRNAs in SGs completely recover for translation following stress relief. Using RNA-seq of translating fractions in human cell line, we found that higher fraction of the m(6)A-modified mRNAs recovered for translation compared to unmodified mRNAs, i.e. 95% vs 84%, respectively. Considering structural mRNA analysis, we found that the m(6)A modification enhances structuring at nucleotides in its close vicinity. Our results suggest that SG-sequestered mRNAs disassemble nearly completely from SGs and the m(6)A modification may display some advantage to the mRNAs in their recovery for translation likely by m(6)A-driven structural stabilization.
引用
收藏
页码：877 / 884
页数：8
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