Internalization, Translocation and Biotransformation of Silica-Coated Titanium Dioxide Nanoparticles in Neural Stem Cells

被引：10

作者：

Wang, Yanli ^{[1
]}

Wang, Jia ^{[1
]}

Wu, Minghong ^{[1
]}

Deng, Xiaoyong ^{[1
]}

Wen, Tieqiao ^{[2
]}

Chen, Chunying ^{[4
]}

Zhang, Haijiao ^{[1
]}

Liu, Yuanfang ^{[1
,3
]}

Jiao, Zheng ^{[1
]}

机构：

[1] Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China

[2] Shanghai Univ, Inst Syst Biol, Shanghai 200444, Peoples R China

[3] Peking Univ, Beijing Natl Lab Mol Sci, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

[4] Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2010年 / 10卷 / 11期

关键词：

Internalization; Translocation; Titanium Dioxide Nanoparticles; Biotransformation; Neural Stem Cells; CARBON NANOTUBES; PARTICLES; TIO2;

D O I：

10.1166/jnn.2010.2824

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanotechnology has gained massive applications in the fields of biology and pharmacology. Recently, more research attention has been paid to explore the interaction between nanoparticles (NPs) and cells, in particular, the entry of NPs into cells. Herein, we focus on the internalization of the FITC-labeled silica-coated titania NPs (FITC-TiO2@SiO2 NPs) in neural stem cells (NSCs) and their subsequent translocation. Various microscopic techniques, such as confocal-laser-scanning microscopy (CFLSM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to track the time-dependent pathway of NPs in cells. It was found that NPs traversed cell membrane and localized around the cell nucleus. Most NPs congregated in lysosomes and were transformed by lysosomes after 48 h co-incubation.

引用

页码：7121 / 7125

页数：5

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