A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins

被引：61

作者：

Bogush, Vladimir G. ^{[1
]}

Sokolova, Olga S. ^{[2
,3
]}

Davydova, Lyubov I. ^{[1
]}

Klinov, Dmitri V. ^{[4
]}

Sidoruk, Konstantin V. ^{[1
]}

Esipova, Natalya G. ^{[5
]}

Neretina, Tatyana V. ^{[4
]}

Orchanskyi, Igor A. ^{[2
]}

Makeev, Vsevolod Yu ^{[1
]}

Tumanyan, Vladimir G. ^{[5
]}

Shaitan, Konstantin V. ^{[2
]}

Debabov, Vladimir G. ^{[1
]}

Kirpichnikov, Mikhail P. ^{[2
]}

机构：

[1] State Sci Ctr GosNIIGenet, Moscow 113545, Russia

[2] Moscow MV Lomonosov State Univ, Fac Biol, Dept Bioengn, Moscow 119991, Russia

[3] RAS, Inst Biochem, Moscow 119071, Russia

[4] RAS, Inst Bioorgan Chem, Moscow 117997, Russia

[5] RAS, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

来源：

JOURNAL OF NEUROIMMUNE PHARMACOLOGY | 2009年 / 4卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

spidroin; 1; and; 2; nanofibrils; electrospinning; antiparallel beta-sheet structure; atomic force microscopy; scanning electron microscopy; CLAVIPES DRAGLINE SILK; AMYLOID FIBRIL; C-13; NMR; FIBROIN; FABRICATION; MICROSCOPY;

D O I：

10.1007/s11481-008-9129-z

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Spider dragline silk possesses impressive mechanical and biochemical properties. It is synthesized by a couple of major ampullate glands in spiders and comprises of two major structural proteins-spidroins 1 and 2. The relationship between structure and mechanical properties of spider silk is not well understood. Here, we modeled the complete process of the spider silk assembly using two new recombinant analogs of spidroins 1 and 2. The artificial genes sequence of the hydrophobic core regions of spidroin 1 and 2 have been designed using computer analysis of existing databases and mathematical modeling. Both proteins were expressed in Pichia pastoris and purified using a cation exchange chromatography. Despite the absence of hydrophilic N- and C-termini, both purified proteins spontaneously formed the nanofibrils and round micelles of about 1 mu m in aqueous solutions. The electron microscopy study has revealed the helical structure of a nanofibril with a repeating motif of 40 nm. Using the electrospinning, the thin films with an antiparallel beta-sheet structure were produced. In summary, we were able to obtain artificial structures with characteristics that are perspective for further biomedical applications, such as producing three-dimensional matrices for tissue engineering and drug delivery.

引用

页码：17 / 27

页数：11

共 52 条

[1] Regeneration of Bombyx mori silk by electrospinning.: Part 3:: characterization of electrospun nonwoven mat [J].