Analysis of human transferrin glycopeptides by capillary electrophoresis and capillary liquid chromatography-mass spectrometry. Application to diagnosis of alcohol dependence

被引：26

作者：

Barroso, Albert ^{[1
]}

Gimenez, Estela ^{[1
]}

Benavente, Fernando ^{[1
]}

Barbosa, Jose ^{[1
]}

Sanz-Nebot, Victoria ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

ANALYTICA CHIMICA ACTA | 2013年 / 804卷

关键词：

Capillary liquid chromatography; Transferrin; Glycopeptides; Glycoforms; Mass spectrometry; Alcohol dependence; ERYTHROPOIESIS-STIMULATING PROTEIN; RECOMBINANT-HUMAN-ERYTHROPOIETIN; INTACT ERYTHROPOIETIN; ENZYMATIC DIGESTION; HUMAN-SERUM; DEPLETION; GLYCAN; ABUSE; TIME; CDT;

D O I：

10.1016/j.aca.2013.09.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, capillary electrophoresis and capillary liquid chromatography coupled to mass spectrometry (CE-TOF-MS and mu LC-TOF-MS) were used to detect and characterise human transferrin (Tf) glycopeptide glycoforms obtained by tryptic digestion. After selecting mu LC-TOF-MS because of improved performance in analysis of N-413 and N-611 glycopeptide glycoforms, the proposed methodology was applied to serum samples. Two immunoaffinity columns were employed to isolate Tf from serum samples. Both columns were activated with the same anti-Tf antibody but using two different bonding chemistries. After immunoaffinity purification and digestion, serum samples from a teetotal individual (as control) and from individuals with low and high alcohol dependence were analysed by mu LC-TOF-MS. Relative abundance of each glycoform was useful to estimate the degree of alcohol dependence of each individual. Finally, the established methodology was used to analyse serum samples from specific individuals with an unknown degree of alcohol dependence. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：167 / 175

页数：9

共 31 条

[1] Modeling the migration behavior of rabbit liver apothioneins in capillary electrophoresis [J].

Benavente, Fernando ;

Andon, Balbina ;

Gimenez, Estella ;

Barbosa, Jose ;

Sanz-Nebot, Victoria .

ELECTROPHORESIS, 2008, 29 (13) :2790-2800

[2] Glycosylation defects underlying fetal alcohol spectrum disorder: a novel pathogenetic model "When the wine goes in, strange things come out" - S.T. Coleridge, The Piccolomini [J].

Binkhorst, M. ;

Wortmann, S. B. ;

Funke, S. ;

Kozicz, T. ;

Wevers, R. A. ;

Morava, E. .

JOURNAL OF INHERITED METABOLIC DISEASE, 2012, 35 (03) :399-405

[3] Carbohydrate-deficient transferrin (CDT) as a marker of alcohol abuse: A critical review of the literature 2001-2005 [J].

Bortolotti, Federica ;

De Paoli, Giorgia ;

Tagliaro, Franco .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 841 (1-2) :96-109

[4] Strategies to study human serum transferrin isoforms using integrated liquid chromatography ICPMS, MALDI-TOF, and ESI-Q-TOF detection:: Application to chronic alcohol abuse [J].

Busto, MED ;

Montes-Bayón, M ;

Blanco-González, E ;

Meija, J ;

Sanz-Medel, A .

ANALYTICAL CHEMISTRY, 2005, 77 (17) :5615-5621

[5] The effects of ethanol on the glycosylation of human transferrin [J].

Flahaut, C ;

Michalski, JC ;

Danel, T ;

Humbert, MH ;

Klein, A .

GLYCOBIOLOGY, 2003, 13 (03) :191-198

[6] Analysis of intact erythropoietin and novel erythropoiesis-stimulating protein by capillary electrophoresis-electrospray-ion trap mass spectrometry [J].

Gimenez, Estela ;

Benavente, Fernando ;

Barbosa, Jose ;

Sanz-Nebot, Victoria .

ELECTROPHORESIS, 2008, 29 (10) :2161-2170

[7] Capillary electrophoresis time-of-flight mass spectrometry for a confident elucidation of a glycopeptide map of recombinant human erythropoietin [J].

Gimenez, Estela ;

Ramos-Hernan, Raquel ;

Benavente, Fernando ;

Barbosa, Jose ;

Sanz-Nebot, Victoria .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (16) :2307-2316

[8] Analysis of recombinant human erythropoietin and novel erythropoiesis stimulating protein digests by immunoaffinity capillary electrophoresis-mass spectrometry [J].

Gimenez, Estela ;

Benavente, Fernando ;

de Bolos, Carme ;

Nicolas, Ernesto ;

Barbosa, Jose ;

Sanz-Nebot, Victoria .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (12) :2574-2582

[9] Denaturation of metalloproteins with EDTA to facilitate enzymatic digestion and mass fingerprinting [J].

Janecki, DJ ;

Reilly, JP .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (10) :1268-1272

[10] Disialo-trisialo bridging of transferrin is due to increased branching and fucosylation of the carbohydrate moiety [J].

Landberg, Eva ;

Astrom, Eva ;

Kagedal, Bertil ;

Pahlsson, Peter .

CLINICA CHIMICA ACTA, 2012, 414 :58-64

← 1 2 3 4 →