A photoelectrochemical aptasensor for thrombin based on the use of carbon quantum dot-sensitized TiO2 and visible-light photoelectrochemical activity

被引：38
作者：
Cheng, Wenjie [1 ,2 ]
Pan, Jiahong [1 ,2 ]
Yang, Jianying [3 ]
Zheng, Zengyao [3 ]
Lu, Fushen [4 ]
Chen, Yaowen [4 ]
Gao, Wenhua [1 ,2 ,4 ]
机构：
[1] Shantou Univ, Dept Chem, Shantou 515063, Guangdong, Peoples R China
[2] Shantou Univ, Lab Preparat & Applicat Ordered Struct Mat Guangd, Shantou 515063, Guangdong, Peoples R China
[3] Natl Detergents & Cosmet Prod Qual Supervis & Ins, Shantou 515041, Guangdong, Peoples R China
[4] Shantou Univ, Anal & Testing Ctr, Shantou 515063, Guangdong, Peoples R China
来源：
MICROCHIMICA ACTA | 2018年 / 185卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Photoelectrochemistry; Aptamer; Nanocomposites; Photoresponse; Photoluminescence; Sensing electrode; LABEL-FREE; SIGNAL AMPLIFICATION; NANODOTS; ARRAYS; APTAMERS; SENSOR; ASSAY;
D O I：
10.1007/s00604-018-2800-z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A photoelectrochemical (PEC) aptasensor for the highly sensitive and specific detection of thrombin is described. This aptasensor is based on an indium tin oxide (ITO) support that is covered with carbon quantum dot (CQD)-sensitized TiO2 and acts as photoactive matrix. The ITO/TiO2/CQD electrode was prepared by impregnation assembly. It displays an enhanced and steady photocurrent response under irradiation by visible light. A carboxyl-functionalized thrombin-binding aptamer was covalently immobilized on the modified ITO to obtain a PEC aptasensor whose photocurrent decreases with increasing concentration of thrombin. Under 420 nm irradiation at a bias voltage of 0 V, the aptasensor has a linear response in the 1.0 to 250 pM thrombin concentration range, with a 0.83 pM detection limit. Conceivably, this approach can be extended to numerous other PEC aptasensors for the detection of targets for which appropriate aptamers are available.
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页数：10
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