Properties of laccases produced by Pycnoporus sanguineus induced by 2,5-xylidine

被引：42

作者：

Garcia, TA

Santiago, MF

Ulhoa, CJ ^{[1
]}

机构：

[1] Univ Fed Goias, Fac Farm, Inst Ciencias Biol, BR-74001970 Goiania, Go, Brazil

[2] Univ Brasilia, Inst Biol, BR-70910900 Brasilia, DF, Brazil

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 09期

关键词：

isoforms characterization; laccase; Pycnoporus sanguineus;

D O I：

10.1007/s10529-006-0026-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C over 5 min reaction. The K-m values of enzymes toward syringaldazine were 10 mu=M (Lac I) and 8 mu M (Lac II). Sodium azide inhibited Lac I (85%) and Lac II (75%) activities.

引用

页码：633 / 636

页数：4

共 21 条

[1] Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus [J].