Exosomes Derived from Mesenchymal Stem Cells Protect the Myocardium Against Ischemia/Reperfusion Injury Through Inhibiting Pyroptosis

被引：39

作者：

Tang, Jiayou ^{[1
]}

Jin, Lu ^{[2
,3
,4
]}

Liu, Yang ^{[1
]}

Li, Lanlan ^{[1
]}

Ma, Yanyan ^{[1
]}

Lu, Linhe ^{[1
]}

Ma, Jipeng ^{[1
]}

Ding, Peng ^{[1
]}

Yang, Xiuling ^{[1
]}

Liu, Jincheng ^{[1
]}

Yang, Jian ^{[1
]}

机构：

[1] Air Force Mil Med Univ, Xijing Hosp, Dept Cardiovasc Surg, 127 Changle West Rd, Xian 710032, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, State Key Lab Mil Stomatol, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China

[3] Fourth Mil Med Univ, Natl Clin Res Ctr Oral Dis, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China

[4] Fourth Mil Med Univ, Shaanxi Clin Res Ctr Oral Dis, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China

来源：

DRUG DESIGN DEVELOPMENT AND THERAPY | 2020年 / 14卷

关键词：

exosome; mesenchymal stem cells; ischemia/reperfusion injury; pyroptosis; miR-320b; ISCHEMIA-REPERFUSION INJURY; INFLAMMASOMES;

D O I：

10.2147/DDDT.S239546

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Objective: Mesenchymal stem cells (MSCs) show unique advantages in cardiomyocyte repairment. Exosomes derived from MSCs can enhance the viability of myocardial cells after ischemia/reperfusion (I/R) injury and regulate inflammation response. The study was designed to ascertain whether MSCs-exo protect the myocardium against I/R injury through inhibiting pyroptosis, and the underlying mechanisms. Methods and Results: Experiments were carried out in H/R and I/R model. Cell viability was inhibited and NLRP3 and caspasel protein levels were upregulated in H/R model. However, MSCs could inhibit cell apoptosis and pyroptosis in H/R model. Moreover, we used MSCs-exo to treated H/R model, and flow cytometric analysis results showed the inhibition function of MSCs-exo on cell apoptosis, and Western blot data suggested that NLRP3 and Caspase-1 expressions were downregulated in H/R model. Furthermore, exosomal miR-320b targeted NLRP3 protein, and MSCs-exo OE could inhibit NLRP3 expression and pyroptosis in H/R. In addition, the inhibition function of MSCs-exo on pyroptosis also was found in I/R model, and HE and Tunel staining also got similar results. Conclusion: Exosomes derived from mesenchymal stem cells could protect the myocardium against ischemia/reperfusion injury through inhibiting pyroptosis.

引用

页码：3765 / +

页数：14

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