Exosomes Derived from Mesenchymal Stem Cells Protect the Myocardium Against Ischemia/Reperfusion Injury Through Inhibiting Pyroptosis

被引：39
作者：
Tang, Jiayou [1 ]
Jin, Lu [2 ,3 ,4 ]
Liu, Yang [1 ]
Li, Lanlan [1 ]
Ma, Yanyan [1 ]
Lu, Linhe [1 ]
Ma, Jipeng [1 ]
Ding, Peng [1 ]
Yang, Xiuling [1 ]
Liu, Jincheng [1 ]
Yang, Jian [1 ]
机构：
[1] Air Force Mil Med Univ, Xijing Hosp, Dept Cardiovasc Surg, 127 Changle West Rd, Xian 710032, Shaanxi, Peoples R China
[2] Fourth Mil Med Univ, State Key Lab Mil Stomatol, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China
[3] Fourth Mil Med Univ, Natl Clin Res Ctr Oral Dis, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China
[4] Fourth Mil Med Univ, Shaanxi Clin Res Ctr Oral Dis, Dept Maxillofacial Plast Surg, Sch Stomatol, Xian, Shaanxi, Peoples R China
来源：
DRUG DESIGN DEVELOPMENT AND THERAPY | 2020年 / 14卷
关键词：
exosome; mesenchymal stem cells; ischemia/reperfusion injury; pyroptosis; miR-320b; ISCHEMIA-REPERFUSION INJURY; INFLAMMASOMES;
D O I：
10.2147/DDDT.S239546
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Objective: Mesenchymal stem cells (MSCs) show unique advantages in cardiomyocyte repairment. Exosomes derived from MSCs can enhance the viability of myocardial cells after ischemia/reperfusion (I/R) injury and regulate inflammation response. The study was designed to ascertain whether MSCs-exo protect the myocardium against I/R injury through inhibiting pyroptosis, and the underlying mechanisms. Methods and Results: Experiments were carried out in H/R and I/R model. Cell viability was inhibited and NLRP3 and caspasel protein levels were upregulated in H/R model. However, MSCs could inhibit cell apoptosis and pyroptosis in H/R model. Moreover, we used MSCs-exo to treated H/R model, and flow cytometric analysis results showed the inhibition function of MSCs-exo on cell apoptosis, and Western blot data suggested that NLRP3 and Caspase-1 expressions were downregulated in H/R model. Furthermore, exosomal miR-320b targeted NLRP3 protein, and MSCs-exo OE could inhibit NLRP3 expression and pyroptosis in H/R. In addition, the inhibition function of MSCs-exo on pyroptosis also was found in I/R model, and HE and Tunel staining also got similar results. Conclusion: Exosomes derived from mesenchymal stem cells could protect the myocardium against ischemia/reperfusion injury through inhibiting pyroptosis.
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页码：3765 / +
页数：14
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