Enhanced Production of Cordycepin by Solid State Fermentation of Cordyceps militaris using Additives

被引:0
作者
Wen, Ting-Chi [1 ]
Kang, Chao [2 ]
Meng, Ze-Bing [1 ]
Qi, Yong-Bin [1 ]
Hyde, Kevin D. [3 ,4 ,5 ]
Kang, Ji-Chuan [1 ]
机构
[1] Guizhou Univ, Minist Educ, Engn & Res Ctr Southwest Biopharmaceut Resources, Guiyang 550025, Guizhou Provinc, Peoples R China
[2] Guizhou Acad Sci, Inst Biol, Guiyang 550009, Guizhou Provinc, Peoples R China
[3] Mae Fah Luang Univ, Inst Excellence Fungal Res, Chiang Rai 57100, Thailand
[4] Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand
[5] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11442, Saudi Arabia
来源
CHIANG MAI JOURNAL OF SCIENCE | 2016年 / 43卷 / 05期
基金
中国国家自然科学基金;
关键词
Cordyceps militaris; cordycepin; solid state fermentation; additives; response surface methodology; FRUITING-BODY FORMATION; CULTURE-CONDITIONS; SUBMERGED CULTURE; OPTIMIZATION; MUSHROOMS;
D O I
暂无
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cordycepin is one of the most important bioactive compounds produced by the insect pathogenic fungus Cordyceps militaris. The effects of medium composition on enhanced production of cordycepin by C. militaris were investigated by changing the liquid supplement medium composition with rice medium in this study. Glucose, peptone, adenine, and histidine were optimized to improve the yield of cordycepin in 300 mL cylindrical glass bottles by solid state fermentation using response surface methodology. The optimum medium for cordycepin production using solid state fermentation were: glucose 26.25 g/L, peptone 26.25 g/L, adenine 7.50 g/L, histidine 4.50 g/L, MgSO4 center dot 7H(2)O 0.1 g/L, and KH2PO4 0.1 g/L in liquid supplement medium. Under these conditions, the maximum cordycepin yield in the rice culture medium was 18.92 mg/g on 39 days, and this value is higher when compared to other reports using solid state fermentation. This is the first report for improving the cordycepin production by adding additives (nucleoside analogue and amino acid) in solid state fermentation by C. militaris. This method provides an effective way for increasing the cordycepin production by solid state fermentation.
引用
收藏
页码:972 / 984
页数:13
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