Monitoring of irritant gas using a whole-cell-based sensor system

被引：8

作者：

Bohrn, U. ^{[1
,2
,3
]}

Stuetz, E. ^{[1
]}

Fuchs, K. ^{[1
]}

Fleischer, M. ^{[1
]}

Schoening, M. J. ^{[2
]}

Wagner, P. ^{[3
]}

机构：

[1] Siemens AG, Corp Res & Technol, D-81739 Munich, Germany

[2] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany

[3] Univ Hasselt, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 175卷

关键词：

Cell-based gas biosensor; Whole-cell sensor; A549; RPMI; 2650; Respiration; Acidification; Impedance; Mid infrared spectroscopy; IN-VITRO EXPOSURE; INDOOR AIR; GASEOUS COMPOUNDS; SENSITIVE LAYER; CULTURE; CYTOTOXICITY; POLLUTANTS; TOXICITY; AMMONIA; BIOSENSORS;

D O I：

10.1016/j.snb.2012.05.088

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas (R)), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：208 / 217

页数：10

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