Fluorescence proteins, live-cell imaging, and mechanobiology: Seeing is believing

被引：195
作者：
Wang, Yingxiao [1 ,2 ]
Shyy, John Y. J. [3 ]
Chien, Shu [4 ,5 ]
机构：
[1] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA
[2] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA
[3] Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA
[4] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[5] Univ Calif San Diego, Whitaker Inst Biomed Engn, La Jolla, CA 92093 USA
来源：
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2008年 / 10卷
关键词：
green fluorescence protein; fluorescence resonance energy transfer; fluorescence recovery after photobleaching; fluorescence lifetime imaging microscopy; chromophore-assisted light inactivation; mechanotransduction;
D O I：
10.1146/annurev.bioeng.010308.161731
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Fluorescence proteins (FPs) have been widely used for live-cell imaging in the past decade. This review summarizes the recent advances in FP development and imaging technologies using FPs to monitor molecular localization and activities and gene expressions in live cells. We also discuss the Utilization of FPs to develop molecular biosensors and the principles and application of advanced technologies such as fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), and chromophore-assisted light inactivation (CALI). We present examples of the application of FPs and biosensors to visualize mechanotransduction events with high spatiotemporal resolutions in live cells. These live-cell imaging technologies, which represent a frontier area in biomedical engineering, can shed new light on the mechanisms regulating mechanobiology at Cellular and molecular levels in normal and pathophysiological conditions.
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页码：1 / 38
页数：38
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共 245 条
  • [1] New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications
    Adams, SR
    Campbell, RE
    Gross, LA
    Martin, BR
    Walkup, GK
    Yao, Y
    Llopis, J
    Tsien, RY
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (21) : 6063 - 6076
  • [2] Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins
    Ai, Hui-wang
    Shaner, Nathan C.
    Cheng, Zihao
    Tsien, Roger Y.
    Campbell, Robert E.
    [J]. BIOCHEMISTRY, 2007, 46 (20) : 5904 - 5910
  • [3] Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein:: structural characterization and applications in fluorescence imaging
    Ai, Hui-wang
    Henderson, J. Nathan
    Remington, S. James
    Campbell, Robert E.
    [J]. BIOCHEMICAL JOURNAL, 2006, 400 (531-540) : 531 - 540
  • [4] Patterns of living β-actin movement in wounded human coronary artery endothelial cells exposed to shear stress
    Albuquerque, MLC
    Flozak, AS
    [J]. EXPERIMENTAL CELL RESEARCH, 2001, 270 (02) : 223 - 234
  • [5] Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity
    Ananthanarayanan, B
    Ni, Q
    Zhang, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (42) : 15081 - 15086
  • [6] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    Ando, R
    Hama, H
    Yamamoto-Hino, M
    Mizuno, H
    Miyawaki, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12651 - 12656
  • [7] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
    Ando, R
    Mizuno, H
    Miyawaki, A
    [J]. SCIENCE, 2004, 306 (5700) : 1370 - 1373
  • [8] Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral
    Baird, GS
    Zacharias, DA
    Tsien, RY
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) : 11984 - 11989
  • [9] Circular permutation and receptor insertion within green fluorescent proteins
    Baird, GS
    Zacharias, DA
    Tsien, RY
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) : 11241 - 11246
  • [10] Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
    Balaban, NQ
    Schwarz, US
    Riveline, D
    Goichberg, P
    Tzur, G
    Sabanay, I
    Mahalu, D
    Safran, S
    Bershadsky, A
    Addadi, L
    Geiger, B
    [J]. NATURE CELL BIOLOGY, 2001, 3 (05) : 466 - 472
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