Transcriptional and Functional Analysis of CD1c+ Human Dendritic Cells Identifies a CD163+ Subset Priming CD8+CD103+ T Cells

被引：233

作者：

Bourdely, Pierre ^{[1
,2
]}

Anselmi, Giorgio ^{[1
,2
]}

Vaivode, Kristine ^{[1
,2
]}

Ramos, Rodrigo Nalio ^{[3
]}

Missolo-Koussou, Yoann ^{[3
]}

Hidalgo, Sofia ^{[3
,4
]}

Tosselo, Jimena ^{[3
]}

Nunez, Nicolas ^{[3
]}

Richer, Wilfrid ^{[3
]}

Vincent-Salomon, Anne ^{[5
]}

Saxena, Alka ^{[6
,7
]}

Wood, Kristie ^{[6
,7
]}

Lladser, Alvaro ^{[4
,8
]}

Piaggio, Eliane ^{[3
]}

Helft, Julie ^{[3
]}

Guermonprez, Pierre ^{[1
,2
,9
]}

机构：

[1] Kings Coll London, Sch Immunol & Microbial Sci, Peter Gorer Dept Immunobiol, Ctr Inflammat Biol & Canc Immunol, London, England

[2] Kings Coll London, Canc Res UK Kings Hlth Partner Canc Ctr, London, England

[3] PSL Res Univ, Inst Curie, Translat Immunotherapy Team, INSERM,Res Ctr,U392, Paris, France

[4] Fdn Ciencia & Vida, Lab Immunooncol, Santiago, Chile

[5] PSL Res Univ, Inst Curie, Dept Biopathol, Paris, France

[6] Guys & St Thomas Hosp, Natl Inst Hlth Res, Biomed Res Ctr, London, England

[7] Kings Coll London, London, England

[8] Univ San Sebastian, Fac Med & Ciencia, Santiago, Chile

[9] Univ Paris, CNRS, Ctr Inflammat Res, INSERM1149,ERL8252, Paris, France

来源：

IMMUNITY | 2020年 / 53卷 / 02期

基金：

英国国家替代、减少和改良动物研究中心; 英国生物技术与生命科学研究理事会;

关键词：

COLONY-STIMULATING FACTOR; MONOCYTE DIFFERENTIATION; GENE-EXPRESSION; GROWTH-FACTOR; FLT3; LIGAND; PROGENITOR; RESIDENT; INTERLEUKIN-4; HETEROGENEITY; MACROPHAGES;

D O I：

10.1016/j.immuni.2020.06.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dendritic cells (DCs) are antigen-presenting cells controlling T cell activation. In humans, the diversity, ontogeny, and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88(-)CD1c(+)CD163(+) DCs (called DC3s) as immediate precursors of inflammatory CD88(-)CD14(+)CD1c(+) CD163(+)Fc epsilon RI+ DCs. DC3s develop via a specific pathway activated by GM-CSF, independent of cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. Like classical DCs but unlike monocytes, DC3s drove activation of naive T cells. In vitro, DC3s displayed a distinctive ability to prime CD8(+) T cells expressing a tissue homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor beta (TGF-beta) signaling. In vivo, DC3s infiltrated luminal breast cancer primary tumors, and DC3 infiltration correlated positively with CD8(+)CD103(+)CD69(+) tissue-resident memory T cells. Together, these findings define DC3s as a lineage of inflammatory DCs endowed with a strong potential to regulate tumor immunity.

引用

页码：335 / +

页数：26

共 64 条

[1] Interleukins 1β and 6 but not transforming growth factor-β are essential for the differentiation of interleukin 17-producing human T helper cells [J].