Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate

被引：67

作者：

Chen, Zhanguang ^{[1
]}

Qian, Sihua ^{[1
]}

Chen, Xi ^{[2
]}

Gao, Wenhua ^{[3
]}

Lin, Yuejuan ^{[3
]}

机构：

[1] Shantou Univ, Dept Chem, Shantou 515063, Peoples R China

[2] Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China

[3] Shantou Univ, Anal & Testing Ctr, Shantou 515063, Peoples R China

来源：

ANALYST | 2012年 / 137卷 / 18期

关键词：

SILVER NANOCLUSTERS; CIRCULAR-DICHROISM; HUMAN URINE; EXTRACTION; METABOLITE; ION;

D O I：

10.1039/c2an35786k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this contribution, bovine serum albumin stabilized gold nanoclusters as novel fluorescent probes were successfully utilized for the detection of methotrexate for the first time. Our prepared gold nanoclusters exhibited strong emission with peak maximum at 633.5 nm. However, the addition of methotrexate induced the strong fluorescence intensity of the gold nanoclusters to decrease. The decrease in fluorescence intensity of the gold nanoclusters caused by methotrexate allowed the sensitive detection of methotrexate in the range of 0.0016 mu g mL(-1) to 24 mu g mL(-1). The detection limit for methotrexate is 0.9 ng mL(-1) at a signal-to-noise ratio of 3. The present sensor for methotrexate detection possessed a low detection limit and wide linear range. In addition, the real samples were analyzed with satisfactory results.

引用

页码：4356 / 4361

页数：6

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