Transcriptional and Chromatin Dynamics of Muscle Regeneration after Severe Trauma

被引：39

作者：

Aguilar, Carlos A. ^{[1
]}

Pop, Ramona ^{[2
,3
]}

Shcherbina, Anna ^{[1
]}

Watts, Alain ^{[1
]}

Matheny, Ronald W., Jr. ^{[4
]}

Cacchiarelli, Davide ^{[2
,3
]}

Han, Woojin M. ^{[5
,6
]}

Shin, Eunjung ^{[6
,7
]}

Nakhai, Shadi A. ^{[6
,7
,8
]}

Jang, Young C. ^{[6
,7
,8
]}

Carrigan, Christopher T. ^{[4
]}

Gifford, Casey A. ^{[2
,3
]}

Kottke, Melissa A. ^{[4
]}

Cesana, Marcella ^{[9
]}

Lee, Jackson ^{[1
]}

Urso, Maria L. ^{[4
]}

Meissner, Alexander ^{[2
,3
]}

机构：

[1] MIT, Lincoln Lab, Lexington, MA 02127 USA

[2] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[3] Harvard Univ, Harvard Stem Cell Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[4] US Army, Mil Performance Div, Inst Environm Med, Natick, MA 01760 USA

[5] Georgia Inst Technol, Woodruff Sch Mech Engn, Atlanta, GA 30332 USA

[6] Georgia Inst Technol, Parker H Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA

[7] Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA

[8] Georgia Inst Technol, Wallace Coulter Dept Biomed Engn, Atlanta, GA 30332 USA

[9] Harvard Med Sch, Boston Childrens Hosp, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

STEM CELL REPORTS | 2016年 / 7卷 / 05期

关键词：

LONG NONCODING RNA; DUCHENNE MUSCULAR-DYSTROPHY; CELL-CYCLE ARREST; SKELETAL-MUSCLE; SATELLITE CELL; STEM-CELLS; INFLAMMATORY MONOCYTES; PI3K P110-ALPHA; MYOD BINDING; DIFFERENTIATION;

D O I：

10.1016/j.stemcr.2016.09.009

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Following injury, adult skeletal muscle undergoes a well-coordinated sequence of molecular and physiological events to promote repair and regeneration. However, a thorough understanding of the in vivo epigenomic and transcriptional mechanisms that control these reparative events is lacking. To address this, we monitored the in vivo dynamics of three histone modifications and coding and non-coding RNA expression throughout the regenerative process in a mouse model of traumatic muscle injury. We first illustrate how both coding and noncoding RNAs in tissues and sorted satellite cells are modified and regulated during various stages after trauma. Next, we use chromatin immunoprecipitation followed by sequencing to evaluate the chromatin state of cis-regulatory elements (promoters and enhancers) and view how these elements evolve and influence various muscle repair and regeneration transcriptional programs. These results provide a comprehensive view of the central factors that regulate muscle regeneration and underscore the multiple levels through which both transcriptional and epigenetic patterns are regulated to enact appropriate repair and regeneration.

引用

页码：983 / 997

页数：15

共 69 条

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