A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ

被引：43

作者：

Zhu, Xiaoli ^{[1
]}

Shi, Hai ^{[1
]}

Shen, Yalan ^{[1
]}

Zhang, Bin ^{[1
]}

Zhao, Jing ^{[1
]}

Li, Genxi ^{[1
,2
]}

机构：

[1] Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China

[2] Nanjing Univ, Dept Biochem, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

来源：

NANO RESEARCH | 2015年 / 8卷 / 08期

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

staining; nucleic acids; polyacrylamide gel; copper nanoclusters; electrophoresis; DSDNA-TEMPLATED FORMATION; ETHIDIUM-BROMIDE; NANOPARTICLES; IDENTIFICATION; STRATEGY; ASSAY; ACID;

D O I：

10.1007/s12274-015-0778-y

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The safety of nucleic acid staining dyes has long been recognized to be a problem. Extensive efforts have been made to search for alternatives to the most popular but toxic staining dye, ethidium bromide (EtBr). However, so far no staining method that can be guaranteed to be sufficiently safe has been developed. In this paper, we report a green staining method of DNA in polyacrylamide gel electrophoresis, where in situ synthesis of DNA-templated fluorescent copper nanoclusters (CuNCs) in the gel is achieved to make the DNA bands visible under UV light. Moreover, a comprehensive study of the performance of this staining method has been conducted and the experimental results show that it has favorable sensitivity, stability, and usability. Meanwhile, in our animal experiments, the two reagents (copper sulfate and ascorbic acid) as well as the synthesized CuNCs have been proven to be non-toxic in contact with skin. In addition, all the reagents employed in this work are readily available and low cost, and the procedure is simple to carry out. Therefore, this novel staining method based on the in situ synthesis DNA-templated fluorescent CuNCs has many potential applications.

引用

页码：2714 / 2720

页数：7

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