Structural basis of microRNA length variety

被引：150

作者：

Starega-Roslan, Julia ^{[1
]}

Krol, Jacek ^{[1
]}

Koscianska, Edyta ^{[1
]}

Kozlowski, Piotr ^{[1
]}

Szlachcic, Wojciech J. ^{[1
]}

Sobczak, Krzysztof ^{[1
]}

Krzyzosiak, Wlodzimierz J. ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, Canc Genet Lab, PL-61704 Poznan, Poland

来源：

NUCLEIC ACIDS RESEARCH | 2011年 / 39卷 / 01期

关键词：

HUMAN DICER; RNA; COMPLEX; TRBP; RECOGNITION; TARGETS;

D O I：

10.1093/nar/gkq727

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biogenesis of human microRNAs ( miRNAs) includes two RNA cleavage steps in which the activities of the RNases Drosha and Dicer are involved. miRNAs of diverse lengths are generated from different genes, and miRNAs that are heterogeneous in length are produced from a single miRNA gene. We determined the solution structures of many miRNA precursors and analysed the structural basis of miRNA length diversity using a new measure: the weighted average length of diced RNA (WALDI). We found that asymmetrical structural motifs present in precursor hairpins are primarily responsible for the length diversity of miRNAs generated by Dicer. High-resolution northern blots of miRNAs and their precursors revealed that both Dicer and Drosha cleavages of imperfect specificity contributed to the miRNA length heterogeneity. The relevance of these findings to the dynamics of the dicing complex, mRNA regulation by miRNA, RNA interference and miRNA technologies are discussed.

引用

页码：257 / 268

页数：12

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