Effects of polarization on the de-excitation dark focal spot in STED microscopy

被引：168
作者：
Hao, Xiang [1 ]
Kuang, Cuifang [1 ]
Wang, Tingting [1 ]
Liu, Xu [1 ]
机构：
[1] Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China
来源：
JOURNAL OF OPTICS | 2010年 / 12卷 / 11期
关键词：
polarization; diffraction theory; fluorescence microscopy; CYLINDRICAL-VECTOR BEAMS; DIFFRACTION; RESOLUTION; PATTERNS; BREAKING; LIGHT; FIELD;
D O I：
10.1088/2040-8978/12/11/115707
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The size of the dark focal spot directly determines the resolution and stability of stimulated emission depletion (STED) microscopy. This paper investigates the relationship between the size of the dark focal spot and the polarization of the input light beam. The types of fundamental polarization are discussed, their effects on the dark focal spot are compared and the optimized mode for each kind of polarization is proposed. The results of the analysis provide the theoretical basis and reference for designing a STED system.
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页数：8
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