Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines
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作者:
Landry, ML
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机构:Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
Landry, ML
Ferguson, D
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机构:Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
Ferguson, D
Cohen, S
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机构:Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
Cohen, S
Peret, TCT
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机构:Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
Peret, TCT
Erdman, DD
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机构:Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
Erdman, DD
机构:
[1] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
[2] Yale New Haven Med Ctr, Dept Lab Med, New Haven, CT 06504 USA
[3] Ctr Dis Control & Prevent, Resp & Enter Viruses Branch, Atlanta, GA USA
Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.