Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines

被引:48
作者
Landry, ML
Ferguson, D
Cohen, S
Peret, TCT
Erdman, DD
机构
[1] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA
[2] Yale New Haven Med Ctr, Dept Lab Med, New Haven, CT 06504 USA
[3] Ctr Dis Control & Prevent, Resp & Enter Viruses Branch, Atlanta, GA USA
关键词
D O I
10.1128/JCM.43.4.1950-1952.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.
引用
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页码:1950 / 1952
页数:3
相关论文
共 11 条
[1]   THE COMPLETE SEQUENCE OF A FULL LENGTH CDNA FOR HUMAN-LIVER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES [J].
ARCARI, P ;
MARTINELLI, R ;
SALVATORE, F .
NUCLEIC ACIDS RESEARCH, 1984, 12 (23) :9179-9189
[2]   Comparative evaluation of real-time PCR assays for detection of the human metapneumovirus [J].
Côté, S ;
Abed, Y ;
Boivin, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3631-3635
[3]   GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness [J].
Erdman, DD ;
Weinberg, GA ;
Edwards, KM ;
Walker, FJ ;
Anderson, BC ;
Winter, J ;
González, M ;
Anderson, LJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) :4298-4303
[4]   A 1-year experience with human Metapneumovirus in children aged <5 years [J].
Esper, F ;
Martinello, RA ;
Boucher, D ;
Weibel, C ;
Ferguson, D ;
Landry, ML ;
Kahn, JS .
JOURNAL OF INFECTIOUS DISEASES, 2004, 189 (08) :1388-1396
[5]   Human metapneumovirus infections in young and elderly adults [J].
Falsey, AR ;
Erdman, D ;
Anderson, LJ ;
Walsh, EE .
JOURNAL OF INFECTIOUS DISEASES, 2003, 187 (05) :785-790
[6]   SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining [J].
Landry, ML ;
Ferguson, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) :708-711
[7]   Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages [J].
Maertzdorf, J ;
Wang, CK ;
Brown, JB ;
Quinto, JD ;
Chu, M ;
de Graaf, M ;
van den Hoogen, BG ;
Spaete, R ;
Osterhaus, ADME ;
Fouchier, RAM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (03) :981-986
[8]   Human metapneumovirus infection among children hospitalized with acute respiratory illness [J].
Mullins, JA ;
Erdman, DD ;
Weinberg, GA ;
Edwards, K ;
Hall, CB ;
Walker, FJ ;
Iwane, M ;
Anderson, LJ .
EMERGING INFECTIOUS DISEASES, 2004, 10 (04) :700-705
[9]  
Navarro-Marí JM, 1999, J CLIN MICROBIOL, V37, P2346
[10]   Characterization of human metapneumoviruses isolated from patients in north America [J].
Peret, TCT ;
Boivin, G ;
Li, Y ;
Couillard, M ;
Humphrey, C ;
Osterhaus, ADME ;
Erdman, DD ;
Anderson, LJ .
JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (11) :1660-1663