Microfluidic tissue model for live cell screening

被引：19

作者：

Lee, Philip J. ^{[1
]}

Gaige, Terry A. ^{[1
]}

Ghorashian, Navid ^{[1
]}

Hung, Paul J. ^{[1
]}

机构：

[1] CellASIC Corp, San Leandro, CA 94577 USA

来源：

BIOTECHNOLOGY PROGRESS | 2007年 / 23卷 / 04期

关键词：

D O I：

10.1021/bp070053l

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a microfluidic platform modeled after the physiologic microcirculation for multiplexed tissue-like culture and high-throughput analysis. Each microfabricated culture unit consisted of three functional components: a 50 mu m wide cell culture pocket, an artificial endothelial barrier with 2,mu m pores, and a nutrient transport channel. This configuration enabled a high density of cancer cells to be maintained for over I week in a solid tumor-like morphology when fed with continuous flow. The microfluidic chip contained 16 parallel units for "flow cell" based experiments where live cells were exposed to a soluble factor and analyzed via fluorescence microscopy or flow-through biochemistry. Each fluidically independent tissue unit contained similar to 500 cells fed with a continuous flow of 10 nL/min. As a demonstration, the toxicity profile of the anti-cancer drug paclitaxel was collected on HeLa cells cultured in the microfluidic format and compared with a 384-well dish for up to 5 days of continuous drug exposure.

引用

页码：946 / 951

页数：6

共 36 条

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