Genetic Polymorphisms of Staphylococcus aureus Isolated from Bovine Mastitis in China

被引:0
作者
Wu, Zhongyong [1 ,2 ,3 ]
Li, Wenzhu [4 ]
Ni, Chunxia [1 ,2 ,3 ]
He, Zhuolin [1 ,2 ,3 ]
Hou, Xiao [1 ,2 ,3 ]
Pu, Wanxia [1 ,2 ,3 ]
机构
[1] CAAS, Key Lab New Anim Drug Project, Lanzhou, Peoples R China
[2] CAAS, Key Lab Vet Pharmaceut Discovery, Minist Agr & Rural Affairs, Lanzhou, Peoples R China
[3] CAAS, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou, Peoples R China
[4] Qilihe Dist Peoples Hosp Lanzhou, Lanzhou, Peoples R China
关键词
Bovine mastitis; Staphylococcus aureus; RAPD; Genetic polymorphisms; STRAINS; MILK; RAPD; PCR;
D O I
10.17582/journal.pjz/20200109070125
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
In order to provide science-based information for the control of cow mastitis, to understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shanghai, Sichuan, and Gansu. The results showed that the amplified product bands with three primers per isolate ranged from 1 similar to 9 and the product sizes from 240 to 4,500 bp. Primer OLP13 had the highest discriminant coefficient. The Staphylococcus aureus strains were divided into 11 genotypes by fingerprint with oligonucleotide primers OLP13. There were 12 strains in type 1, 8 in each of types 2 and 5, 22 in each of types 3 and 7, 13 in types 4, 11 in type 6, 25 in type 8, 28 in type 9, 8 in type 10 and 5 in type 11. Among the 11 types of S. aureus isolates, type3 (14/37) and type 7 (15/35) were the main prevalent strains in Guizhou and Inner Mongolia Province respectively. Type 9 (21/55) had the highest prevalence rate and type 8 (10/55) was higher than other types in Shanghai, both of them were closely related. The results showed that the genotype distribution in various regions was noticeably different. Differences in dairy farming practices, geographical environments, and the use of antibiotics may be the reasons for the varied distribution of different genotypes in those provinces.
引用
收藏
页码:2417 / 2425
页数:9
相关论文
共 22 条
[1]   Molecular techniques for detecting and typing of bacteria, advantages and application to foodborne pathogens isolated from ducks [J].
Adzitey, Frederick ;
Huda, Nurul ;
Ali, Gulam Rusul Rahmat .
3 BIOTECH, 2013, 3 (02) :97-107
[2]  
Boriollo M.F.G., 2018, BRAZ ORAL SCI, V16, P1, DOI [10.20396/bjos.v16i0.8650497, DOI 10.20396/BJOS.V16I0.8650497]
[3]  
Cui YingPeng Cui YingPeng, 2007, China Tropical Medicine, V7, P1753
[4]   Identification and antimicrobial suceptibility profile of bacteria causing bovine mastitis from dairy farms in Pelotas, Rio Grande do Sul [J].
Freitas, C. H. ;
Mendes, J. F. ;
Villarreal, P. V. ;
Santos, P. R. ;
Goncalves, C. L. ;
Gonzales, H. L. ;
Nascente, P. S. .
BRAZILIAN JOURNAL OF BIOLOGY, 2018, 78 (04) :661-666
[5]  
Hamid S, 2017, Vet World, V10, P363, DOI 10.14202/vetworld.2017.363-367
[6]   Prevalence and molecular characterization of Staphylococcus aureus from human stool samples [J].
Kates, A. E. ;
Thapaliya, D. ;
Smith, T. C. ;
Chorazy, M. L. .
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 2018, 7
[7]   Molecular Characteristics of Staphylococcus aureus Causing Bovine Mastitis between 2014 and 2015 [J].
Li, Tianming ;
Lu, Huiying ;
Wang, Xing ;
Gao, Qianqian ;
Dai, Yingxin ;
Shang, Jun ;
Li, Min .
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2017, 7
[8]   Udder infections with Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis at calving in dairy herds with suboptimal udder health [J].
Lundberg, A. ;
Nyman, A-K. ;
Aspan, A. ;
Borjesson, S. ;
Unnerstad, H. Ericsson ;
Waller, K. Persson .
JOURNAL OF DAIRY SCIENCE, 2016, 99 (03) :2102-2117
[9]   Genetic procedures for identification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks [J].
Martín, MC ;
Fueyo, JM ;
González-Hevia, MA ;
Mendoza, MC .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 94 (03) :279-286
[10]  
Mostafa S.I., 2013, AFRI J MICROBIOL RES, V7, P755