Modulation of the Human Pancreatic Ductal Adenocarcinoma Immune Microenvironment by Stereotactic Body Radiotherapy

被引：41

作者：

Mills, Bradley N. ^{[1
]}

Qiu, Haoming ^{[2
,3
]}

Drage, Michael G. ^{[4
]}

Chen, Chunmo ^{[4
]}

Mathew, Jocelyn S. ^{[1
]}

Garrett-Larsen, Jesse ^{[1
]}

Ye, Jian ^{[1
]}

Uccello, Taylor P. ^{[5
]}

Murphy, Joseph D. ^{[5
]}

Belt, Brian A. ^{[1
]}

Lord, Edith M. ^{[3
,5
]}

Katz, Alan W. ^{[2
,3
]}

Linehan, David C. ^{[1
,3
]}

Gerber, Scott A. ^{[1
,3
,5
]}

机构：

[1] Univ Rochester, Dept Surg, Med Ctr, 601 Elmwood Ave, Rochester, NY 14642 USA

[2] Univ Rochester, Dept Radiat Oncol, Med Ctr, Rochester, NY 14642 USA

[3] Univ Rochester, Wilmot Canc Inst, Med Ctr, Rochester, NY 14642 USA

[4] Univ Rochester, Dept Pathol & Lab Med, Med Ctr, Rochester, NY 14642 USA

[5] Univ Rochester, Dept Microbiol & Immunol, Med Ctr, Rochester, NY 14642 USA

来源：

CLINICAL CANCER RESEARCH | 2022年 / 28卷 / 01期

关键词：

RADIATION-THERAPY; TUMOR MICROENVIRONMENT; CELL-DEATH; BLOCKADE; CANCER; EXPRESSION; SIGNATURES; APOPTOSIS; RADIOSURGERY; MACROPHAGES;

D O I：

10.1158/1078-0432.CCR-21-2495

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose: Stereotactic body radiotherapy (SBRT) is an emerging treatment modality for pancreatic ductal adenocarcinoma (PDAC), which can effectively prime cytotoxic T cells by inducing immu-nogenic tumor cell death in preclinical models. SBRT effects on human PDAC have yet to be thoroughly investigated; therefore, this study aimed to characterize immunomodulation in the human PDAC tumor microenvironment following therapy. Experimental Design: Tumor samples were obtained from patients with resectable PDAC. Radiotherapy was delivered a median of 7 days prior to surgical resection, and sections were analyzed by multiplex IHC (mIHC), RNA sequencing, and T-cell receptor sequencing (TCR-seq). Results: Analysis of SBRT-treated tumor tissue indicated reduced tumor cell density and increased immunogenic cell

引用

页码：150 / 162

页数：13

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