A simple lysosome-targeted probe for detection of hypochlorous acid in living cells

被引：8

作者：

Jiao, Chunpeng ^{[1
,2
]}

Liu, Yuanyuan ^{[1
,2
]}

Pang, Jingxiang ^{[3
,4
]}

Lu, Wenjuan ^{[3
,4
]}

Zhang, Pingping ^{[3
,4
]}

Wang, Yanfeng ^{[3
,4
]}

机构：

[1] Univ Jinan, Shandong Acad Med Sci, Sch Med & Life Sci, Jinan 250200, Shandong, Peoples R China

[2] Shandong Acad Med Sci, Inst Mat Media, Key Lab Rare & Uncommon Dis Shandong Prov, Minist Hlth,Key Lab Biotech Drugs, Jinan, Shandong, Peoples R China

[3] Shandong First Med Univ, Jinan 250062, Shandong, Peoples R China

[4] Shandong Acad Med Sci, Jinan 250062, Shandong, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2020年 / 392卷 / 392期

基金：

中国国家自然科学基金;

关键词：

Hypochlorous acid; Fluorescent probe; Living cells; Lysosome-targetable; RATIOMETRIC FLUORESCENT-PROBE; SELECTIVE DETECTION; NITRIC-OXIDE; MITOCHONDRIA;

D O I：

10.1016/j.jphotochem.2020.112399

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Since HOCl plays an indispensable role in a variety of physiological and pathological processes, monitoring the level of hypochlorous acid (HOCl) in living cells is critical. Based on this, it is necessary to develop a convenient and simple technique to detect HOCl in living cells. In this contribution, a simple 2-hydroxy-6-morpholino-1H-benzo[de]isoquinoline-1,3(2 H)-dione (NDS) was developed as fluorescent probe for monitoring HOCl in live cells. Probe NDS exhibited preeminent specificity towards HOCl over other various bioactive substances. In addition, the probe could respond to HOCl in solutions within 16 s and the limit of detection was as low as 105.24 nM. NDS has good lysosomal targeting and has been successfully applied to the expression of exogenous and endogenous lysosomal HOCl in HeLa cells. This study provides a promising tool for studying physiological or pathological related processes.

引用

页数：7

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