PNA solubility enhancers

被引：56

作者：

Gildea, BD ^{[1
]}

Casey, S ^{[1
]}

MacNeill, J ^{[1
]}

Perry-O'Keefe, H ^{[1
]}

Sorensen, D ^{[1
]}

Coull, JM ^{[1
]}

机构：

[1] Boston Probes Inc, Bedford, MA 01730 USA

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 40期

关键词：

D O I：

10.1016/S0040-4039(98)01581-0

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Fmoc-protected hydrophilic amino acids were prepared and incorporated into peptide nucleic acid (FNA). N- and C-terminal modification of FNA did not affect its ability to hybridize to complementary nucleic acid targets as determined by dot blot, PNA-FISH and Tm experiments. The compounds allowed us to prepare several probes which were previously impossible to make, purify or use due to limited aqueous solubility or self-aggregation. (C) 1998 Elsevier Science Ltd. All rights reserved.

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页码：7255 / 7258

页数：4

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