Bone morphogenetic protein 2 promotes transforming growth factor β3-induced chondrogenesis of human osteoarthritic synovium-derived stem cells

被引：22

作者：

Rui Yun-feng ^{[1
,3
,4
]}

Du Lin ^{[2
]}

Wang You ^{[1
]}

Wang Yang ^{[1
]}

Lui Pauline po-yee ^{[3
,4
,5
]}

Tang Ting-ting ^{[1
,2
]}

Chan Kai-ming ^{[3
,4
,5
]}

Dai Ke-rong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Orthopaed, Shanghai 200011, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Key Lab Orthopaed Implant, Shanghai 200011, Peoples R China

[3] Chinese Univ Hong Kong, Fac Med, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Fac Med, Hong Kong Jockey Club Sports Med & Hlth Sci Ctr, Hong Kong, Hong Kong, Peoples R China

[5] Chinese Univ Hong Kong, Sch Biomed Sci, Program Stem Cell & Regenerat, Hong Kong, Hong Kong, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2010年 / 123卷 / 21期

关键词：

chondrogenesis; synovial membrane; mesenchymal stem cell; osteoarthritis; bone morphogenetic protein 2; transforming growth factor beta3; VITRO CARTILAGE FORMATION; MARROW STROMAL CELLS; EXPRESSION; ALGINATE; DIFFERENTIATION; MEMBRANE; TISSUE; REGENERATION; SYNOVECTOMY; AGGRECAN;

D O I：

10.3760/cma.j.issn.0366-6999.2010.21.017

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Synovium-derived stem cells (SDSCs) with higher chondrogenic potential are attracting considerable attention as a cell source for cartilage regeneration. We investigated the effect of bone morphogenetic protein 2 (BMP-2) on transforming growth factor beta3 (TGF-beta 3)-induced chondrogenesis of SDSCs isolated from human osteoarthritic synovium in a pellet culture system. Methods The clonogenicity, stem cell marker expression and multi-differentiation potential of isolated SDSCs were determined by colony forming unit assay, flow cytometry and specific staining including alizarin red S, Oil red 0 and alcian blue staining, respectively. SDSCs pellet was cultured in chondrogenic medium with or without TGF-beta 3 or/and BMP-2. At day 21, the diameter and the weight of the pellets were measured. Chondrogenic differentiation of SDSCs was evaluated by Safranin 0 staining, immunohistochemical staining of collagen type II, sulfated glycosaminoglycan (sGAG) synthesis and mRNA expression of collagen type II, aggrecan, SOX9, link-protein, collagen type X and BMP receptor II. Results Cells isolated under the optimized culturing density (10(4)/60 cm(2)) showed clonogenicity and multi-differentiation potential. These cells were positive (>99%) for CD44, CD90, CD105 and negative (<10%) for CD34 and CD71. SDSCs differentiated to a chondrocytic phenotype in chondrogenic medium containing TGF-beta 3 with or without BMP-2. Safranin 0 staining of the extracellular matrix was positive and the expression of collagen type II was detected. Cell pellets treated with TGF-beta 3 and BMP-2 were larger in diameter and weight, produced more sGAGs, and expressed higher levels of collagen type II and other chondrogenic markers, except COL10A1, than medium with TGF-beta 3 alone. Conclusions SDSCs could be isolated from human osteoarthritic synovium. Supplementation with BMP-2 significantly promoted the in vitro TGF-beta 3-induced chondrogenic differentiation of SDSCs. Chin Med J 2010;123(21):3040-3048

引用

页码：3040 / 3048

页数：9

共 41 条

[1] Cartilage repair using an in vitro generated scaffold-free tissue-engineered construct derived from porcine synovial mesenchymal stem cells [J].