Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China

被引：19

作者：

Wang Wei ^{[1
,2
]}

Liu Feng ^{[1
,3
]}

Zulqarnain, Baloch ^{[4
]}

Zhang Cun Shan ^{[5
]}

Ma Ke ^{[1
]}

Peng Zi Xin ^{[2
]}

Yan Shao Fei ^{[2
]}

Hu Yu Jie ^{[2
]}

Gan Xin ^{[2
]}

Dong Yin Ping ^{[2
]}

Bai Yao ^{[2
]}

Li Feng Qin ^{[2
]}

Yan Xiao Mei ^{[6
]}

Ma Ai Guo ^{[1
]}

Xu Jin ^{[2
]}

机构：

[1] Qingdao Univ, Sch Publ Hlth, Qingdao 266071, Shandong, Peoples R China

[2] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China

[3] Qingdao Hiser Med Ctr, Pharmaceut Dept, Qingdao 266033, Shandong, Peoples R China

[4] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

[5] Kuiwen Dist Ctr Dis Control & Prevent, Weifang 261000, Shandong, Peoples R China

[6] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2017年 / 30卷 / 08期

关键词：

Methicillin-resistant Staphylococcus aureus; Antimicrobial susceptibility; Genetic diversity; Pig; Retail food; GEL-ELECTROPHORESIS PROTOCOLS; LIVESTOCK-ASSOCIATED MRSA; ANTIMICROBIAL RESISTANCE; BOVINE MASTITIS; PREVALENCE; STRAINS; SUSCEPTIBILITY; EPIDEMIOLOGY; HOSPITALS; PROFILE;

D O I：

10.3967/bes2017.076

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance (MDR). Greater diversity was found in food-associated MRSA (7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA (3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates (40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone (96.4%), and CC9-ST9-t437-MC621 (20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains (t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.

引用

页码：570 / 580

页数：11