Thin-Layer Chromatographic Specification and Separation of Cu1+, Cu2+, Ni2+, and Co2+ Cations

The M(PyDTC)2 (M: Cu, Co, or Ni) and CuPyDTC complexes, prepared by reactions of ammonium pyrrolidinedithiocarbamate with metal nitrates, are examined for qualitative analysis, speciation, and mutual separation using thin-layer chromatography systems. These complexes and their mixtures are spotted to the activated and non-activated thin layers of silica gel 60GF 254 (Si-60GF254) with a 250-μm thickness. Toluene-dichloromethane mixtures (4:1, 1:1, 1:4 v/v) are used as mobile phases for running of the complexes. All of these chromatographic systems are successfully used for speciation of Cu2+ and Cu1+ cations. The best analytical separation for the qualitative analysis of corresponding metal cations and mutual separation of components in M(PyDTC)2 and CuPyDTC complexes are obtained when using pure toluene-dichloromethane (1:1 v/v) on the activated layer. This study shows that it is possible to qualitatively analyze and satisfactorily separate a mixture of Cu1+, Cu 2+, Ni2+, and Co2+ cations on cited chromatographic systems. These results may be also said for the adaptability or validity on column chromatography.