Gut mucosal microbiota profiles linked to colorectal cancer recurrence

被引:40
作者
Huo, Rui-Xue [1 ]
Wang, Yi-Jia [2 ]
Hou, Shao-Bin [3 ]
Wang, Wei [4 ]
Zhang, Chun-Ze [5 ,6 ]
Wan, Xue-Hua [4 ,7 ]
机构
[1] Nankai Univ, Tianjin Union Med Ctr, Dept Oncol, Tianjin 300121, Peoples R China
[2] Nankai Univ, Tianjin Union Med Ctr, Lab Oncol Mol Med, Tianjin 300121, Peoples R China
[3] Univ Hawaii Manoa, Adv Studies Genom Prote & Bioinformat, Honolulu, HI 96822 USA
[4] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
[5] Nankai Univ, Tianjin Union Med Ctr, Dept Colorectal Surg, Tianjin 300121, Peoples R China
[6] Tianjin Inst Coloproctol, Tianjin 300121, Peoples R China
[7] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, 23 Hongda St, Tianjin 300457, Peoples R China
基金
国家重点研发计划;
关键词
Gut microbiota; Colorectal cancer; Prognosis; Colorectal cancer recurrence; Biomarker; 16S rRNA sequencing analysis; FUSOBACTERIUM-NUCLEATUM; FECAL MICROBIOTA; COLON-CANCER; GEN; NOV; ASSOCIATION; SURVIVAL; BACTERIA; DRIVER; RISK;
D O I
10.3748/wjg.v28.i18.1946
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
BACKGROUND Emerging evidence links gut microbiota to various human diseases including colorectal cancer (CRC) initiation and development. However, gut microbiota profiles associated with CRC recurrence and patient prognosis are not completely understood yet, especially in a Chinese cohort. AIM To investigate the relationship between gut mucosal microbiota profiles and CRC recurrence and patient prognosis. METHODS We obtained the composition and structure of gut microbiota collected from 75 patients diagnosed with CRC and 26 healthy controls. The patients were followed up by regular examination to determine whether tumors recurred. Triplet-paired samples from on-tumor, adjacent-tumor and off-tumor sites of patients diagnosed with/without CRC recurrence were analyzed to assess spatial-specific patterns of gut mucosal microbiota by 16S ribosomal RNA sequencing. Next, we carried out bioinformatic analyses, Kaplan-Meier survival analyses and Cox regression analyses to determine the relationship between gut mucosal microbiota profiles and CRC recurrence and patient prognosis. RESULTS We observed spatial-specific patterns of gut mucosal microbiota profiles linked to CRC recurrence and patient prognosis. A total of 17 bacterial genera/families were identified as potential biomarkers for CRC recurrence and patient prognosis, including Anaerotruncus, Bacteroidales, Coriobacteriaceae, Dialister, Eubacterium, Fusobacterium, Filifactor, Gemella, Haemophilus, Mogibacteriazeae, Pyramidobacter, Parvimonas, Porphyromonadaceae, Slackia, Schwartzia, TG5 and Treponema. CONCLUSION Our work suggests that intestinal microbiota can serve as biomarkers to predict the risk of CRC recurrence and patient death.
引用
收藏
页码:1946 / 1964
页数:19
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