Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation

被引：0
作者：
Ashman, R
Crimeen-Irwin, B
Ludford-Menting, M
Russell, S
Gu, M
机构：
[1] Swinburne Univ Technol, Ctr Microphoton, Sch Biophys Sci & Elect Engn, Hawthorn, Vic 3122, Australia
[2] Peter MacCallum Canc Res Inst, Trescowthick Res Labs, Melbourne, Vic 3000, Australia
来源：
SCANNING | 2003年 / 25卷 / 03期
关键词：
two-photon microscopy; lifetime imaging; fluorescence resonance energy transfer;
D O I：
暂无
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
We show two-photon spectra and lifetimes acquired using conventional confocal microscopes equipped with an ultra-short pulsed laser and a time-gated intensified charge coupled device. We report on the two-photon spectra and lifetimes of Alexa350, enhanced green fluorescent protein (EGFP), EGFP-CD46, and Cy3 labelled antibodies. Cellular and extracellular EGFP two-photon spectra and lifetimes are compared.
引用
收藏
页码：116 / 120
页数：5
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